Fig 1: LRRC15 induces MMPs to mediate migration and invasion. (A) Western blot analysis was used to analyse protein expression levels of LRRC15 in CAFs and NFs. (B) Western blot analysis was used to detect protein expression of α-SMA in CAFs and NFs. **P<0.01 vs. NFs. (C) Western blotting showed LRRC15 protein expression after transfection with pcDNA3.1-LRRC15 or siLRRC15 into CAFs. CAFs transfected with pcDNA3.1-LRRC15 plasmids or siLRRC15 were used to treat triple-negative breast cancer MDA-MB-231 and MDA-MB-468 cells. (D) The effect of LRRC15 on the migration and invasion of MDA-MB-231 and MDA-MB-468 cells were detected using Transwell assays. (E) Effect of LRRC15 on the protein expression levels of MMPs was detected by western blotting. **P<0.01 vs. Vector group; ##P<0.01 vs. siNC group. LRRC15, leucine-rich repeat-containing protein 15; CAFs, cancer-associated fibroblasts; NFs, normal fibroblasts; α-SMA, α-smooth muscle actin; si, small interfering RNA; NC, negative control.
Fig 2: High LRRC15 expression is associated with poor prognosis in patients with TNBC. (A) The LRRC15 expression data of 235 TNBC and 208 normal samples from TCGA database. (B) The LRRC15 expression in the different TNM stages of patients from TCGA database. Kaplan-Meier analysis of (C) OS, (D) DSS, (E) DFI and (F) PFI from TCGA database. **P<0.01. LRRC15, leucine-rich repeat-containing protein 15; TNBC, triple-negative breast cancer; TCGA, The Cancer Genome Atlas; OS, overall survival; DSS, disease-specific survival; DFI, disease-free interval; PFI, progression-free interval; HR, hazard ratio; CI, confidence interval.
Fig 3: LRRC15 induces migration and invasion via Wnt/β-catenin signalling. The migratory and invasive abilities of MDA-MB-231 and MDA-MB-468 cells were observed after the addition of 10 µmol/l XAV939. The effects of LRRC15 on the migration and invasion of MDA-MB-231 and MDA-MB-468 cells were detected via Transwell assays. **P<0.01 vs. Vector group; ##P<0.01 vs. LRRC15 group. LRRC15, leucine-rich repeat-containing protein 15.
Fig 4: LRRC15 promotes β-catenin levels by downregulating the destruction complex protein Axin1. (A) Effect of LRRC15 on the expression of Axin1, GSK-3β and p-GSK-3β (Ser9) protein expression levels were detected via western blotting. (B) Effect of LRRC15 on the expression of cyclin D1 and c-myc protein expression levels was detected via western blotting. *P<0.05, **P<0.01 vs. Vector group; #P<0.05, ##P<0.01 vs. siNC group. LRRC15, leucine-rich repeat-containing protein 15; p-, phosphorylated; si, small interfering RNA; NC, negative control.
Fig 5: LRRC15 promotes β-catenin levels and transcriptional activity. (A) Effect of LRRC15 on the expression of β-catenin, p-β-catenin (Ser33/37) and β-TrCP protein levels were detected via western blotting. (B) Western blotting results showed that LRRC15 overexpression increased nuclear translocation of β-catenin and knockdown of LRRC15 had the opposite function. (C) Effect of LRRC15 on β-catenin/T cell factor/lymphoid enhancer binding factor-mediated transcriptional activity. *P<0.05, **P<0.01 vs. Vector group; #P<0.05, ##P<0.01 vs. siNC group. LRRC15, leucine-rich repeat-containing protein 15; p-, phosphorylated; β-TrCP, β-transducin repeats-containing proteins; si, small interfering RNA; NC, negative control.
Supplier Page from Abcam for Anti-LRRC15 antibody