Fig 1: The association of CHL1 with PDCD6 depends on Ca2+. (A) Homogenates of brains from 3‐day‐old CHL1+/+ mice were subjected to immunoprecipitation (IP) with rabbit IgG control (ctrl) or PDCD6 antibody (PDCD6) followed by western blot analysis using antibodies against CHL1 and PDCD6. (B) PBS control (ctrl) or His‐tagged CHL1‐ICD was incubated with brain homogenates of 3‐day‐old CHL1+/+ mice followed by pull‐down with Ni‐NTA beads. Brain homogenates (input) and immunoprecipitates were probed by western blot analysis with antibodies against PDCD6, His‐tag, and PEF1 (as negative control). (C) PBS control (ctrl) or his‐tagged CHL1‐ICD was incubated with brain homogenates of 3‐day‐old CHL1+/+ mice containing PBS alone (−) or PBS and 0.5 mM EGTA (EGTA), followed by pull‐down with Ni‐NTA beads. Brain homogenates (input) and precipitates were probed by western blot using antibody against PDCD6. Precipitates were probed by western blot analysis using antibody against His‐tag for the quantification of CHL1‐ICD. Experiments were performed independently three times