Fig 1: Depletion of hPXR increases the expression of genes related to xenobiotic metabolism. (A) Volcano plot of RNA-seq data showing differential expression of hPXR KO versus WT control HepaRG cells. UGT2B15, UGT2B10, UGT2B4, CYP3A4, CYP2C9 and CYP2B6 are among the highly upregulated genes (red) following functional knockout of hPXR. Downregulated genes are shown in blue. Genes with adjusted P-values (adj. P-value) of =0.05 or having |log2(fold-change)| values =1 are shown in gray. Fold change, hPXR KO over wild type (WT) HepaRG cells. (B) Western blot of CYP3A4 and CYP2C9 proteins in WT and hPXR KO cells. ß-Actin was used as the protein loading control.
Fig 2: Protein expression of CYP3A4 (50 kDa) as well as CYP2C9 (56 kDa) (a, b) and the ratio of the gray value of the target band to the gray value of the β-actin band (c, d) for each group, ∗P < 0.05 and ∗∗P < 0.01.
Supplier Page from Abcam for Anti-CYP2C9 antibody [EPR7340]