Fig 1: Kaplan-Meier survival plots. (A) Disease-free and (B) overall survival curves are shown. TAZ-high cases demonstrated less favorable disease-free and overall survival. TAZ, transcriptional coactivator with PDZ-binding motif.
Fig 2: TAZ expression in normal endometrial and endometrioid adenocarcinoma tissues. (A) Limited signals representing TAZ expression were detected in non-cancerous endometrial tissues (magnification, ×200). (B) Tumor cells with a solid structure (right of image) expressed high levels of TAZ compared with cells with a glandular structure (left of image) (magnification, ×400). (C) Portions invading into the myometrium expressed high levels of TAZ (magnification, ×40). (D) High-power field of (C) (magnification, ×200). Invading tumor cells (right of image) expressed high levels of TAZ compared with non-invading cells (left of image). TAZ, transcriptional coactivator with PDZ-binding motif.
Fig 3: Knockdown of transcriptional coactivator with PDZ binding motif (TAZ) inhibits migration and invasion of hepatocellular carcinoma cell lines. (a, b) Migration and invasion of stable TAZ knockdown cells were measured by Transwell assay. Results were quantitated by counting migrated and invasive cells in five randomly chosen high-power fields for each replicate (n = 3). (c) Effects of TAZ knockdown on MMP-2 and MMP-9 were measured by Western blot. Data was expressed as mean ± SD. *P < 0.05. si-NC, non-targeting siRNA; si-TAZ, TAZ siRNA.
Fig 4: Knockdown of transcriptional coactivator with PDZ binding motif (TAZ) inhibits cancer cell proliferation. (a, b) Bel-7402 and HCC-LM3 hepatocellular carcinoma cell lines were transfected with non-targeting siRNA (NC) or TAZ siRNA, and the expression of TAZ was measured by real-time PCR and Western blot after stable cell lines were selected. (c) Cell proliferation was measured by CCK-8 assay after cells were incubated for 1, 2, 3, and 4 days (n = 8). (d) Effects of TAZ knockdown on stem cell markers (OCT4, Nanog, and SOX2) were measured by RT-PCR and Western blot. ß-actin was used as a positive control. Data was expressed as mean ± SD. *P < 0.05.
Fig 5: Transcriptional coactivator with PDZ binding motif (TAZ) knockdown reduces epithelial–mesenchymal transition (EMT) of hepatocellular carcinoma cell lines. (a) mRNA levels of EMT marker genes such as E-cadherin, ß-catenin, N-cadherin, Slug, Snail, and vimentin were measured by RT-PCR. Data are expressed as mean ± SD. *P < 0.05. (b) EMT marker protein levels were measured by Western blot. ß-actin was used as a positive control. Data are expressed as mean ± SD. *P < 0.05. si-NC, non-targeting siRNA; si-TAZ, TAZ siRNA.
Supplier Page from Abcam for Anti-TAZ antibody