Fig 1: Western blots for DBC2-associated components from reticulocyte lysates identified by LC-MS/MS.Flag-tagged DBC2 was synthesized and immuno-adsorbed from RRL with anti-Flag antibody-agarose, washed with P100T, and analyzed by SDS-PAGE, and Western blotting for DBC2 and co-adsorption of Cul3 and CSN4 as described under Materials and methods.
Fig 2: Model for the modulation of DBC2 GTP binding/activation by the Hsp90 chaperone machine.The domains of DBC2 domains are colored light green (Rho domain), gray (BTB1 domain), blue (BTB2 domain), and brown (putative RING domain). Hsp90-dependent binding of Cul3 (red) and CSN4 (brown) and other COP9 signalosome components (yellow-green) are used as example of DBC2-associating proteins. “Early”, “intermediate”, and “late” refer to complexes that are formed during Hsp90's ATPase cycle.
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