Fig 1: Representative immunoblots of USH1 protein expression in USH1 fibroblasts: A total of 30 µg of protein was subjected to SDS/PAGE. Representative immunoblots showing the expression of different USH1 proteins in the indicated radioresistant control and USH1 fibroblasts. Expression of USH1B (A–C), USH1C (D), USH1D (E), and USH1G (F) in total protein extracts. Expression of USH1B (G), USH1C (H), and USH1G/SANS (I) in cytoplasmic protein extracts. It is noteworthy that the blot was cut to provide panel I.
Fig 2: Subcellular localization of USH1 proteins in USH1 and control fibroblasts: Anti-USH1B/MYO7A, anti-USH1D/CDH23, anti-USH1C/Harmonin, and anti-USH1G/SANS immunofluorescence was applied to the radioresistant 149BR controls and the USH1 GM03891 fibroblasts, either before or after irradiation (2 Gy + 24 h), as indicated. The white bar represents 5 µm.
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