Fig 1: Phosphorylation of LIMK2 and CFL1 in IL-4 treated HCAEC.(A) pLIMK2 (pT505) and pCFL1 (pCofilin-1, pS3) were immunostained (red) in HCAEC treated with IL-4 either alone or in combination with Y-27632. F-actin (fluorescent phalloidin, green) and nuclei (DAPI, blue) were also stained. Photomicrographs were acquired using a Zeiss Axioskope equipped with AxioCam MRm digital camera and AxioVision Rel.4.6 software. Original magnification, 200×. (B) Mean cellular fluorescent intensities of pLIMK2 and pCFL1. Data represent mean ± SD from five different regions. *P<0.05. The experiment was repeated three times, with analogous results. (C, D) Immunoblots of pLIMK2, LIMK2, pCFL and CFL in HCAEC treated with IL-4 either alone or in combination with Y-27632. Left band, non-treated cells; middle band, IL-4 treated cells; right band, IL-4+Y-27632 treated cells. Data are mean ± SEM from three independent experiments. *P<0.05.
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