Fig 1: Expression efficiency of KAI/CD82 following stable selection of cells. Expression of GFP indicates the overexpression of KAI1/CD82 in stable cells selected from the transfected 293T cells (top row) and OSCC-15 cells (bottom row). Images were captured at magnification, ×10.
Fig 2: Effect of the overexpression of KAI1 on xenograft tumor growth. The OSCC-15 cells, vector or KAI1/CD82-overexpressing cells were injected into the right rear flank of nude mice. Tumor volumes were recorded for 25 days following inoculation. (A) Tumors excised from three mice in each group. (B) Tumor volumes. P<0.05. (C) Tumor inhibitory rate in the nude mice. P<0.05. Data are presented as the mean ± standard deviation (n=3).
Fig 3: Effect of the expression of KAI/CD82 on apoptosis. The apoptotic rates of OSCC-15, OSCC-15+NC and OSCC-15+KAI1/CD82 cells were determined using Annexin V/PI staining. (A) Flow cytometric analysis; upper right quadrants represent late apoptotic cells; lower right quadrants represent early apoptotic cells. (B) Percentages of early apoptotic cells are shown on the upper graph, total cell apoptosis (late+early apoptosis) in the three groups is shown on the lower graph. Data are presented as the mean ± standard deviation (n=3). *P<0.05, vs. OSCC-15 group; *P<0.05, vs. OSCC-15 + NC group.
Fig 4: Effect of the expression of KAI1 on the in vitro invasion of OSCC-15 cells. (A) Representative images of the three groups of cells, which invaded through the Matrigel; images were captured under a light microscope (magnification, ×200). (B) Quantification of the number of invading cells in each group. Data are presented as the mean ± standard deviation (n=3). *P<0.05, vs. OSCC-15 group and negative control group.
Fig 5: Detection of the protein expression of KAI1/CD82 in transfected 293T cells. (A) Protein levels of KAI1/CD82 were detected using western blot analyses in 293T cells at different time points (0, 24, 48 and 72 h) following transfection. (B) Expression of KAI1/CD82 was normalized by the expression of GAPDH. Data are presented as the mean ± standard deviation (n=3). *P<0.05.
Supplier Page from Abcam for Anti-CD82 antibody [C33]