Anti-S6K1 antibody [E343] ab32529 from Abcam

Product Specs
ItemAnti-S6K1 antibody [E343]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32529
Antibody TypePrimary
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
IsotypeIgG
HostRabbit
CloneE343
ClonalityMonoclonal
Conjugate/TagUnconjugated
Gene NameRPS6KB1
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetS6K1

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Abcam

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United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Palmatine treats urticaria by reducing inflammation and increasing autophagyFrontiers in ImmunologyNovember 14, 2023Tian Xiao, Xingzhi Yu, Liping Yang, Xiaohua DuanProintech), AMPK (1:1000; 5831, Proteintech), p-AMPK (1:1000), p70S6K1 (1:5000 ratio), p-p70S6K1 (1:500; ab32529, Abcam, Cambridge, UK), Atg5-Atg12 complex (1:1000; AAM79 Bio-Rad, CA, USA), or GAPDH (1:1000). The

(2) Activation of acetyl-CoA synthetase 2 mediates kidney injury in diabetic nephropathyJCI InsightOctober 23, 2023Jian Lu, Xue Qi Li, Pei Pei Chen, Jia Xiu Zhang, Liang Liu, Gui Hua Wang, Xiao Qi Liu, Ting Ting Jiang, Meng Ying Wang, Wen Tao Liu, et al.control. The following primary antibodies were used: anti-ACSS2 (ab133664), anti-p62 (ab56416), anti-S6K1 (ab32529), and anti–p-S6K1 (ab59208) purchased from Abcam; anti-nephrin (sc-376522) purchased from Santa Cruz Biotechnology

(3) The amino acid metabolomics signature of differentiating myocardial infarction from strangulation death in mice modelsScientific ReportsSeptember 11, 2023Song-Jun Wang, Bing-Rui Liu, Fu Zhang, Xiao-Rui Su, Ya-Ping Li, Chen-Teng Yang, Zhi-Hua Zhang, Bin Congdatabase. Immunohistochemical analyses Anti-AMPKα1 (ab32047), Cyt-C (ab133504), mTOR (ab109268), PPM1K (ab135286), and S6K1 (ab32529) (Abcam, Cambridge, UK) antibodies were applied in 1:100 dilutions at 4°C for 12 h according to the

(4) Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cellsMolecular Medicine ReportsAugust 9, 2023Xinyan Pan, Chunqiao Liu, Xing Wang, Ming Zhao, Zhimei Zhang, Xuemei Zhang, Chao Wang, Guangyao Songantibody, 1:1,000; p-mTOR (cat. no. ab109268; Abcam), rabbit antibody, 1:1,000; t-p70S6K (cat. no. ab32529; Abcam), rabbit antibody, 1:1,000; p-p70S6K (cat. no. ab59208; Abcam), rabbit antibody, 1:1,000; β-actin

(5) KLF5‑mediated expression of CARD11 promotes the progression of gastric cancerExperimental and Therapeutic MedicineJuly 17, 2023Qiusen Li, Sheng Li, Zongxian Li, Hongyan Xu, Wenxian Zhang(1:1,000; cat. no. ab59208; Abcam), mTOR (1:10,000; cat. no. ab134903; Abcam), P70S6K (1:5,000; cat. no. ab32529; Abcam) and GAPDH (1:2,500; cat. no. ab9485; Abcam) at 4˚C overnight; the following day, the membrane

(6) Celastrol-regulated gut microbiota and bile acid metabolism alleviate hepatocellular carcinoma proliferation by regulating the interaction between FXR and RXRα in vivo and in vitroFrontiers in PharmacologyFebruary 15, 2023Dequan Zeng, Lipen Zhang, Qiang LuoCambridge, UK",Ab32028 Anti-p-mTOR,"Abcam, Cambridge, UK",Ab109268 Anti-S6K1,"Abcam, Cambridge, UK",Ab32529 Anti-p-S6K1,"Abcam, Cambridge, UK",Ab59208 Anti-p-Rb,"Abcam, Cambridge, UK",Ab184796 Anti-myc,"Abcam,

(7) Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagyNature CommunicationsDecember 1, 2022Jingyi Li, Tao Zhang, Tao Ren, Xiaoyu Liao, Yilong Hao, Je Sun Lim, Jong-Ho Lee, Mi Li, Jichun Shao, Rui Liu(ab97695, 1:1000), PRMT5 (ab109451, 1:1000), KDM5C (ab194288, 1:1000), BNIP3 (ab109362, 1:1000), S6K (ab32529, 1:1000), ACC (ab109368, 1:1000), ACC pS79 (ab68191, 1:500), ULK1 pS757 (ab229909, 1:500), and ULK1

(8) Electroacupuncture induces weight loss by regulating tuberous sclerosis complex 1-mammalian target of rapamycin methylation and hypothalamic autophagy in high-fat diet-induced obese ratsFrontiers in PharmacologyOctober 12, 2022Junpeng Yao, Xiangyun Yan, Xianjun Xiao, Xi You, Yanqiu Li, Yuqing Yang, Wei Zhang, Ying LiSQSTM1/p62 (ab109012, 1:10000, Abcam), mTOR (ab134903, 1:10000, Abcam), p-mTOR (5536S, 1:1000, CST), p70S6K (ab32529, 1:5000, Abcam), and p-p70S6K (9234S, 1:1000, CST). The membrane was incubated with the secondary antibodies

(9) Upregulation of KIF18B facilitates malignant phenotype of esophageal squamous cell carcinoma by activating CDCA8 / mTORC1 pathwayJournal of Clinical Laboratory AnalysisOctober 1, 2022Jiangliu Xie, Bo Wang, Wenjie Luo, Chen Li, Xunchao JiaCDK4 (ab108357, 34 kDa, Abcam, UK, 1:1000), CyclinD3 (ab28283, 35 kDa, Abcam, UK, 1:1000), p70S6K (ab32529, 70 kDa, Abcam, UK, 1:5000), phosphorylated (p)‐p70S6K (ab59208, 60 kDa, Abcam, UK, 1:1000), p‐mTOR

(10) Epstein-Barr viral product-containing exosomes promote fibrosis and nasopharyngeal carcinoma progression through activation of YAP1/FAPα signaling in fibroblastsJournal of experimental & clinical cancer research : CRAugust 20, 2022Lee PJ, Sui YH, Liu TT, Tsang NM, Huang CH, Lin TY, Chang KP, Liu SC.ab92726), GAPDH (Abcam, ab9484), active YAP1 (Abcam, ab205270), mTOR (Abcam, ab2732), p70S6K1 (Abcam, ab32529), HSP70 (System Biosciences, EXOAB-HSP70A-1), β-actin (Sigma, A5060), α-tubulin (Santa Cruz Biotechnology,

(11) Modulating autophagic flux via ROS-responsive targeted micelles to restore neuronal proteostasis in Alzheimer's diseaseBioactive materialsMay 1, 2022Xu S, Yang P, Qian K, Li Y, Guo Q, Wang P, Meng R, Wu J, Cao J, Cheng Y, et al.anti-Cathepsin D antibody (1: 2000 dilution, ab75852, Abcam), Anti-S6K1 antibody (1: 2500 dilution, ab32529, Abcam), anti-p-S6K1(Thr389) antibody (1:1000 dilution, 9234, CST), anti-4EBP1 (1:1000 dilution, ab32024,

(12) FGF21 alleviates pulmonary hypertension by inhibiting mTORC1/EIF4EBP1 pathway via H19Journal of Cellular and Molecular MedicineMay 1, 2022Xiuchun Li, Yaxin Zhang, Lihuang Su, Luqiong Cai, Chi Zhang, Jianhao Zhang, Junwei Sun, Mengyu Chai, Mengsi Cai, Qian Wu, et al.ab270967, Abcam), rabbit anti‐phospho‐S6K (Thr389, 1:1000, #9234, Abcam), rabbit anti‐S6K (1:1000, ab32529, Abcam), rabbit anti‐phospho‐mTOR (Ser2448, 1:1000, ab109268, Abcam), rabbit anti‐mTOR (1:1000, ab2732,

(13) xCT contributes to colorectal cancer tumorigenesis through upregulation of the MELK oncogene and activation of the AKT/mTOR cascadeCell Death & DiseaseApril 19, 2022Bufu Tang et al.WB:1:1000, IF: 1:200), CD133 (ab216323, Flow Cyt: 1:100), MELK (ab273015, WB:1:1000, IF: 1:200), S6K (ab32529, WB:1:1000), P-S6K (ab131436, WB:1:1000), β-Actin (ab8226, WB:1:4000) were purchased from Abcam (Cambridge,

(14) MicroRNA‐100 Enhances Autophagy and Suppresses Migration and Invasion of Renal Cell Carcinoma Cells via Disruption of NOX4‐Dependent mTOR PathwayClinical and Translational ScienceFebruary 1, 2022Xiumin Liu, Lili Zhong, Ping Li, Peng Zhao(ab48394, 1: 1000; Abcam), MMP‐2 (ab37150, 1: 1000; Abcam), MMP‐9 (ab73734, 1: 1000; Abcam), S6K1 (ab32529, 1: 5000; Abcam), and glyceraldehyde 3‐phosphate dehydrogenase ABP57259, 1: 5000; Abbkine Scientific,

(15) Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signalingiScienceJanuary 21, 2022Akusjärvi SS, Ambikan AT, Krishnan S, Gupta S, Sperk M, Végvári Á, Mikaeloff F, Healy K, Vesterbacka J, Nowak P, et al.Akt(S473) (Abcam, #ab81283), mTOR (Abcam, #ab32028), mTOR(S2448) (Abcam, #ab109268), S6K1 (Abcam, #ab32529), S6K1(T389 + T412) (Abcam, #ab60948), 4EBP1 (Abcam, #ab32024), 4EBP1(T37) (Abcam, #ab75767), or β-Actin

(16) circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling PathwayJournal of Healthcare EngineeringJanuary 6, 2022Yu Li, Guangle Qin, Jinyun Du, Peng Yue, Yanling Zhang, Na Hou(1 : 1000, rabbit, ab32028), p-S6K1 (phospho-S424, 1 : 500, rabbit, ab131436), S6K1 (1 : 5000, rabbit, ab32529), p-4EBP (1 : 500, rabbit, ab47365), and 4EBP (1 : 2000, rabbit, ab32024) (Abcam, USA) were added and

(17) CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1‑mediated Warburg effectOncology LettersJanuary 5, 2022Wenyu Yang, Yanchun Wang, Chunhui Tao, Yunhai Li, Shan Cao, Xiqian Yangtranslation initiation factor 4E (EIF-4E, 1:5,000, ab32024), p-S6K (1:1,000, ab60948), S6K (1:5,000, ab32529), p-S6 (1:10,000, ab184551), and S6 (1:1,000, ab32132), purchased from Abcam (Cambridge, UK), followed

(18) Angiotensin 1–7 prevents the excessive force loss resulting from 14- and 28-day denervation in mouse EDL and soleus muscleJournal of General PhysiologyDecember 6, 2021Hind Albadrani, T. Ammar, Michael Bader, Jean-Marc RenaudCell Signaling Technology), rabbit anti-MuRF-1 (catalog no. 554561AP; Cedarlane), rabbit anti-total S6 (catalog no. Ab32529; Abcam), rabbit anti-Atrogin-1 (catalog no. AP2041; ECM Bioscience), mouse anti-phospho-S6 (P-S6; catalog

(19) Se improves GPX4 expression and SOD activity to alleviate heat-stress-induced ferroptosis-like death in goat mammary epithelial cellsAnimal Cells and SystemsNovember 8, 2021Lu Liu, Manjiang Wang, Ning Gong, Peng Tian, Hongxia Dengtotal mTOR (t-mTOR, 1:10000, Abcam, ab134903), p-S6K1 (1:500, Abcam, ab131436), t-S6K1 (1:5000, Abcam, ab32529), p-4EBP1 (1:1000, Abcam, ab75767), t-4EBP1 (1:1000, Abcam, ab32130) and β-tubulin (1:5000, Abcam, ab7291)

(20) TEOA Promotes Autophagic Cell Death via ROS-Mediated Inhibition of mTOR/p70S6k Signaling Pathway in Pancreatic Cancer CellsFrontiers in Cell and Developmental BiologyOctober 6, 2021Chen Yang et al.anti-mTOR (Abcam ab134903) (1:1,000), anti-p-p70S6K (Abcam ab131436) (1:1,000), anti-p70S6K (Abcam ab32529) (1:1,000), anti-p-S6 (CST 4858S) (1:2,000), anti-S6 (CST 2217s) (1:1,000), anti-p-4EBP1 (CST 9459S)

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Anti-S6K1 antibody [E343] from Abcam

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Anti-S6K1 antibody [E343] from Abcam

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