Anti-PTEN antibody [Y184] ab32199 from Abcam

Product Specs
ItemAnti-PTEN antibody [Y184]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab32199
Antibody TypePrimary
Quantity10 UL, 40 UL, 100 UL
CloneY184
ApplicationsWestern blot
HostRabbit
ClonalityMonoclonal
IsotypeIgG
Conjugate/TagUnconjugated
Gene NamePTEN
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetPTEN

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Sub-anaesthetic dose of propofol attenuates mechanical allodynia in chronic post-ischaemic pain via regulation of PTEN/PI3K/IL-6 signallingMolecular PainDecember 1, 2023Siu Yi Doreen Leung, Fei Meng, Jingjing Liu, Aijia Jessica Liu, Hei Lui Lhotse Ng, Chi Wai Cheung, Sau Ching Stanley Sau Ching Wongwith primary antibodies at 4°C overnight. The primary antibodies used are anti-PTEN [Y184] (Abcam #ab32199, 1:200), anti-phosphoPTEN (Abcam #ab195056, 1:150), anti-phospho-PI3Kp85α (Abcam #ab182651, 1:400),

(2) Triple‑negative breast cancer cells that survive ionizing radiation exhibit an Axl‑dependent aggressive radioresistant phenotypeExp Ther MedAugust 3, 2023Jianfeng Ji, Yuqin Ding, Yue Kong, Min Fang, Xiaofu Yu, Xiaojing Lai, Qing GuAbcam), Akt (cat. no. ab38499; 1:1,000; Abcam), mTOR (cat. no. ab2732; 1:2,000; Abcam), PTEN (cat. no. ab32199; 1:5,000; Abcam), or GAPDH (cat. no. ab8245; 1:2,000; Abcam), followed by incubation with the relevant

(3) Interactions of EGFR/PTEN/mTOR-Pathway Activation and Estrogen Receptor Expression in Cervical CancerJournal of Personalized MedicineJuly 26, 2023Thomas Bartl, Christoph Grimm, Robert M. Mader, Christoph Zielinski, Gerald Prager, Matthias Unseld, Merima Herac-KornauthCell Signaling, clone: 2976 49F9, dilution 1:50), and anti-PTEN monoclonal antibody (source: Abcam, clone: Ab32199, Cambridge, UK, dilution 1:50) according to the manufacturer specifications (Supplementary Figure S1a–f).

(4) In Situ Nitric Oxide Gas Nanogenerator Reprograms Glioma Immunosuppressive MicroenvironmentAdvanced ScienceJune 1, 2023Yang Liu, Lin Cui, Xiao Wang, Weiling Miao, Yongxu Ju, Tiandong Chen, Huiting Xu, Ning Gu, Fang Yangab150078), anti p62 antibody (Abcam; catalogue no: ab109012), anti PTEN antibody (Abcam; catalogue no: ab32199), anti‐Calreticulin antibody (Abcam; catalogue no: ab22683), anti GAPDH antibody (Abcam; catalogue no:

(5) GATA6-AS1 via Sponging miR-543 to Regulate PTEN/AKT Signaling Axis Suppresses Cell Proliferation and Migration in Gastric CancerMediators of InflammationMay 26, 2023Yi Jin, Daqing Jiangpolyvinylidene fluoride (PVDF) membranes. The diluted primary antibodies against PTEN (1 : 10,000, ab32199; Abcam) and loading control GAPDH (1 : 1,000, ab8245; Abcam) were incubated with the blocked membranes

(6) Deficiency of MicroRNA-23-27-24 Clusters Exhibits the Impairment of Myelination in the Central Nervous SystemNeural PlasticityMarch 22, 2023Yuji Tsuchikawa, Naosuke Kamei, Yohei Sanada, Toshio Nakamae, Takahiro Harada, Kazunori Imaizumi, Takayuki Akimoto, Shigeru Miyaki, Nobuo AdachiRRID:AB_10973392), rabbit anti-phosphatase and tensin homolog deleted on chromosome 10 (PTEN, 1 : 500, ab32199, Abcam, RRID:AB_777535), rabbit anti-lamin B1 (1 : 1000, ab16048 Abcam, RRID:AB_10107828), rabbit anti-LZTR1

(7) miR-19-3p Targets PTEN to Regulate Cervical Cancer Cell Proliferation, Invasion, and AutophagyGenetics ResearchMarch 3, 2023Wei Wang, Lu Liu, Yongjian Tian(4240GR025, Biofroxx) for 2 h at room temperature, the membranes were incubated with anti-PTEN (1 : 1000, ab32199, Abcam) and anti-GAPDH (1 : 1000. ab9485, Abcam) antibodies at 4°C overnight. After washing 3 times

(8) Nanoparticles (NPs)-mediated systemic mRNA delivery to reverse trastuzumab resistance for effective breast cancer therapyActa Pharmaceutica Sinica BMarch 1, 2023Zhihui Dong, Zhuoshan Huang, Senlin Li, Ying Wang, Yandan Yao, Xianzhu Yang, Xiaoding Xu(Cy5-EGFP mRNA and Cy5-PTEN mRNA) were purchased from APExBIO (Houston, TX, USA). PTEN rabbit mAb (#ab32199), HER2 rabbit mAb (ab214275), Ki67 rabbit mAb (#ab92742), and GAPDH rabbit mAb (#ab181602) were purchased

(9) LncRNA LINC00534 regulates cell proliferation and migration via the miR‐494‐3p/PTEN axis in HTR‐8/SVneo cellsJournal of Clinical Laboratory AnalysisJanuary 1, 2023Xuejing Song, Xinxin Zhang, Qing Xia, Chunyan Li, Yanrong Zhang, Yu Huang, Li Meng, Chunhua Wang, Jingyun Li, Wei Long1:1000, ab32537), NF–κB p65 (CST, 1:1000, #8242), STAT3 (Abcam, 1:2000, ab68153), PTEN (Abcam, 1:5000, ab32199), and β‐ACTIN (Proteintech, 1:1000, 66009‐1‐lg) overnight at 4°C. The membranes were washed three times

(10) Epigallocatechin-3-O-gallate promotes extracellular matrix and inhibits inflammation in IL-1β stimulated chondrocytes by the PTEN/miRNA-29b pathwayPharmaceutical BiologyDecember 31, 2022Dong Yang, Guanghua Cao, Xiaorong Ba, Haibo Jiang(C)-caspase-3,Rabbit anti-cleaved caspase-3 antibody,ab2302,Abcam,1:500 PTEN,Rabbit anti-PTEN antibody,ab32199,Abcam,"1:10,000" GAPDH,Rabbit anti-GAPDH antibody,ab181602,Abcam,"1:10,000" List of primary antibodies

(11) Mesenchymal stem cell-originated exosomal circDIDO1 suppresses hepatic stellate cell activation by miR-141-3p/PTEN/AKT pathway in human liver fibrosisDrug DeliveryDecember 31, 2022Li Ma, Junfeng Wei, Yanli Zeng, Junping Liu, Erhui Xiao, Yuehua Kang, Yi Kangab109201), CD63 (1:2000, ab68418), α-SMA (1:2000, ab5694), collagen I (1:2000, ab34710), PTEN (1:10,000, ab32199), and β-actin (1:1000, ab6276), were obtained from Abcam (Cambridge, UK), and protein kinase B (AKT)

(12) Effect of CTP-mediated PTEN on 5637 bladder cancer cells and the underlying molecular mechanismBMC UrologyDecember 10, 2022Bei Yu, Yuan Huang, Yue Yang, Haifeng Hu, Jin Yangpurchased from Invitrogen Corporation. Rabbit anti-PTEN monoclonal antibody was purchased from Abcam (ab32199). Goat anti-rabbit IgG-HRP was purchased from Santa Cruz Biotechnology, Inc. 5637 bladder cancer cells

(13) SIRT1 restoration enhances chondrocyte autophagy in osteoarthritis through PTEN-mediated EGFR ubiquitinationCell Death DiscoveryDecember 1, 2022Qunshan Lu, Peilai Liu, Zhuang Miao, Desu Luo, Songlin Li, Mei LuDiluted primary antibodies of anti-SIRT1 (1:1000, ab110304, Abcam, Cambridge, UK), anti-PTEN (1:1000, ab32199, Abcam), anti-Aggrecan (1:1000, ab36861, Abcam), anti-Collagen II (1:1000, ab34712, Abcam), anti-MMP-13

(14) Morin Inhibits Dox-Induced Vascular Inflammation By Regulating PTEN/AKT/NF-κB PathwayInflammationDecember 1, 2022Yu J, Qi HL, Zhang H, Zhao ZY, Jing-Zhao, Nie ZY.(1:1000, Proteintech, 21,865–1-AP), anti-TNF-α (1:1000, Proteintech, 6029–1-Ig), anti-PTEN (1:1000, Abcam, ab32199), anti-pan-AKT (1:1000, Abcam, ab8805), anti-pan-AKT (phospho T308; 1:1000, Abcam, ab38449), and 1:1000

(15) Morphine-mediated release of astrocyte-derived extracellular vesicle miR-23a induces loss of pericyte coverage at the blood-brain barrier: Implications for neuroinflammationFrontiers in Cell and Developmental BiologyNovember 21, 2022Ke Liao, Fang Niu, Guoku Hu, Shilpa Buchfollowed by an incubation of antibodies specific hnRNP A2/B1 (1:1000; sc-53531; Santa Cruz), PTEN (1:1000; ab32199; Abcam), p-Akt (1:1000; 9271S; Cell Signaling), Akt (1:1000; 9272S; Cell Signaling), GAPDH (1: 5000,

(16) Potential pathogenic mechanism of type 1 X-linked lymphoproliferative syndrome caused by a mutation of SH2D1A gene in an infant: A case reportMedicine (Baltimore)October 14, 2022Yanchun Wang, Yan Wang, Weimin Lu, Lvyan Tao, Yang Xiao, Yuantao Zhou, Xiaoli He, Yu Zhang, Li LiAnti-PI3 Kinase p110δ (Abcam, ab109006); Anti-PI3 Kinase p85α (Abcam, ab191606); Anti-PTEN (Abcam, ab32199); Anti-AKT (CST, 9272s); Anti-phospho-AKT (CST, 9271s); Anti-mTOR (Abcam, ab2732). 2.5. Prediction

(17) Cross Brain–Gut Analysis Highlighted Hub Genes and LncRNA Networks Differentially Modified During Leucine Consumption and Endurance Exercise in Mice with Depression-Like BehaviorsMolecular NeurobiologyJuly 1, 2022Navid Abedpoor, Farzaneh Taghian, Fatemeh Hajibabaie162–0176, USA). The primary antibodies used were rabbit KDR antibody (Abcam, ab11939, USA), anti-PTEN (ab32199, Abcam), and mouse anti-GAPDH antibody (Santa Cruz, sc-32233). Membranes were incubated with primary

(18) Repeated cell sorting ensures the homogeneity of ocular cell populations expressing a transgenic proteinPLOS ONEMarch 25, 2022Tsan-Chi Chen, Shu-Wen ChangSanta Cruz Biotechnology, Santa Cruz, CA), GFP (sc-8334; rabbit polyclonal; Santa Cruz Biotechnology), PTEN (ab32199; rabbit monoclonal; Abcam), Thy-1 (ab133350; rabbit monoclonal; Abcam), or ZO-1 (ab96587; rabbit monoclonal;

(19) The phosphatase and tensin homolog gene inserted between NP and P gene of recombinant New castle disease virus oncolytic effect test to glioblastoma cell and xenograft mouse modelVirology journalJanuary 29, 2022Jang SH, Jung BK, An YH, Jang H.Waltham, MA, USA). Blocking was performed by 1% BSA for 1 h. PTEN monoclonal antibody (1:1000 cat. No. ab32199 Abcam, Cambridge, United Kingdom), Phospho-AKT1 (Ser473) monoclonal antibody (1:1000 cat. No. 44-621G

(20) Identification of a Cowden syndrome patient with a novel PTEN mutation and establishment of patient-derived induced pluripotent stem cellsIn Vitro Cellular & Developmental Biology - AnimalJanuary 3, 2022Fumitaka Obayashi, Atsuko Hamada, Sachiko Yamasaki, Taku Kanda, Shigeaki Toratani, Tetsuji OkamotoAntibody,Cat. no,Company PTEN,ab32199,Abcam p-PTEN(Ser380),#9511,Cell Signaling Technology AKT,sc-5298,SANTA CRUZ p-AKT(Ser437),#9271,Cell Signaling Technology

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Anti-PTEN antibody [Y184] from Abcam

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Anti-PTEN antibody [Y184] from Abcam

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