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Antibodies
Anti-F-actin antibody [4E3.adl]
Anti-F-actin antibody [4E3.adl] from Abcam
Be the first to write a review!
Citations:
(29)
Supplier Page
Supplier Page from
Abcam for
Anti-F-actin antibody [4E3.adl]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-F-actin antibody [4E3.adl]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab130935
Quantity
10 UG, 100 UG, 100 UL
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Conjugate/Tag
Unconjugated
Clonality
Monoclonal
Isotype
IgM
Clone
4E3.adl
Host
Mouse, Nude mouse
Antibody Type
Primary
Gene Name
ACTA1
Reactivity
Human, Mouse
Target
F-actin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (29)
(1)
Silencing Glypican-1 enhances the antitumor effects of Pictilisib via downregulating PI3K/Akt/ERK signaling in chemo-resistant esophageal adenocarcinoma
Molecular & Cellular Oncology
December 31, 2023
Akshay Pratap, Andrea Qualman, Hedlund Garrett, Lindsey Westbrook, Erlinda The, Sanchayita Mitra, Mila Cordero, Kenneth Meza Monge, Juan- Pablo Idrovo, Argudit Chauhan,
et al.
Signaling,
1:1000
ERK1/2,Cell Signaling,Cell Signaling,
1:1000
AKT
,
Cell Signaling
,
4691
,
1:1000
F-Actin,
Abcam
,
ab130935
,
1:1000
SNAIL 1,Proteintec,13099–1-AP,
1:1000
Vimentin,Proteintec,10366-AP,
1:1000
Beta Actin,Proteintec,66009–1-Ig,"
1:10,000
" Secondary
(2)
Inhibition of TGF-β2–Induced Trabecular Meshwork Fibrosis by Pirfenidone
Translational Vision Science & Technology
November 17, 2023
Xiaofeng Zhu, Bei Zeng, Caiqing Wu, Zidong Chen, Minbin Yu, Yangfan Yang
No."
Anti-myocilin
,
1:2000
,—,"
Proteintech
, 60357" Anti-α-SMA,
1:10000
,
1:500
,"
Abcam
,
ab124964
" Anti-F-actin,
1:500
,
1:500
,"
Abcam
,
ab130935
" Anti-collagen IV,
1:1000
,
1:500
,"
Abcam
,
ab6586
" Anti-fibronectin,
1:1000
,
1:500
,"
Abcam
,
ab2413
" Primary
(3)
ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance
Discover Oncology
October 24, 2023
Chao Xu, Yongjie Xie, Peng Xie, Jianming Li, Zhongsheng Tong, Yanfang Yang
incubated with primary antibodies against
ZDHHC9
(Proteintech,
24046-1-AP
,
1:100
) and F-actin (
Abcam
,
ab130935
,
1:200
) overnight at 4 ℃. The following day, the cells were incubated with fluorescently labelled secondary
(4)
hucMSCs treatment prevents pulmonary fibrosis by reducing circANKRD42-YAP1-mediated mechanical stiffness
Aging (Albany NY)
June 16, 2023
Haitong Zhang, Qi Zhu, Yunxia Ji, Meirong Wang, Qian Zhang, Weili Liu, Ruiqiong Li, Jinjin Zhang, Pan Xu, Xiaodong Song,
et al.
(
Cell Signaling Technology
,
14074S
), anti-phospho-
YAP1
(
Cell Signaling Technology
,
53749S
), anti-F-actin (
Abcam
,
ab130935
), anti-
GAPDH
(
Affinity
,
AF7021
). 1×Tris buffered saline Tween was used to wash the
membranes
for three
(5)
The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues
Graefe's Archive for Clinical and Experimental Ophthalmology
May 1, 2023
Rossella Colabelli Gisoldi, Gemma Lodato, Bijorn Omar Balzamino, Graziana Esposito, Alessandra Micera, Augusto Pocobelli
1:1000
;
sc-374429
;
Santa Cruz
Biotech, Santa Cruz, CA), F-actin (
anti-F-actin antibody, [4E3.adl
],
ab130935
; predicted/observed band size at 42/37 kDa;
1:1000
;
Abcam
, Cambridge, UK), and VEGF (anti-VEGF 165A
(6)
The Slingshot phosphatase 2 is required for acrosome biogenesis during spermatogenesis in mice
eLife
March 21, 2023
Xu K, Su X, Fang K, Lv Y, Huang T, Li M, Wang Z, Yin Y, Muhammad T, Liu S,
et al
.
dilution,
sc-365414
,
Santa Cruz Biotechnology
),
mouse
anti-F-ACTIN monoclonal antibody (
1:500
dilution,
ab130935
,
Abcam
), rabbit anti-BAX antibody (
1:1000
dilution,
2772
,
Cell Signaling Technology
), rabbit anti-BAD
(7)
Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia
Iran J Basic Med Sci
January 1, 2023
Huisi Lin, Yujuan Fan, Zhifu Zhi, Lihong Pang, Dan Sun
(
ab33186
), rabbit monoclonal anti-cortactin antibody (
ab81208
), and primary antibody-like anti-F-actin (
ab130935
) were obtained from
Abcam
(Cambridge, MA, USA), while the primary antibody anti-Rac1 (bs-4186R) was
(8)
Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis
Cardiovascular Diabetology
December 1, 2022
Rongjun Zou, Wanting Shi, Junxiong Qiu, Na Zhou, Na Du, Hao Zhou, Xinxin Chen, Li Ma
Drp1
(#
ab184247
),
Fis1
(#
ab156865
), GAPDH (#
ab8245
),
Tom20
(#
ab186735
), DNA-PKcs (#
ab32566
), F-actin (#
ab130935
) and Met (#ab51067). The following antibodies were obtained from
Cell Signaling Technology
: p-Drp1 (#
4494
),
(9)
Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis
Cell Communication and Signaling
December 1, 2022
Siyuan Yin, Ru Song, Jiaxu Ma, Chunyan Liu, Zhenjie Wu, Guoqi Cao, Jian Liu, Guang Zhang, Huayu Zhang, Rui Sun,
et al.
anti-YAP
(#
14074
), and anti-
CREB
(#
9197
), all diluted
1:200
and purchased from
CST
, and
rat
anti-Actin (#
ab130935
) was diluted
1:200
and purchased from
Abcam
. CUT & RUN
assay
Protein-DNA interactions were analysed
(10)
Transfer of microRNA-22-3p by M2 macrophage-derived extracellular vesicles facilitates the development of ankylosing spondylitis through the PER2-mediated Wnt/β-catenin axis
Cell Death Discovery
December 1, 2022
Chong Liu, Tuo Liang, Zide Zhang, Jiarui Chen, Jang Xue, Xinli Zhan, Liang Ren
4′,6-diamidino-2-phenylindole (DAPI) (blue), after which the
cytoskeleton
was stained green using F-actin (
ab130935
,
Abcam
). Then, the cells were incubated at 37 °C for 12 h. The uptake of M2-EVs by cells was visualized
(11)
Endothelial Barrier Disruption by Lipid Emulsions Containing a High Amount of N3 Fatty Acids (Omegaven) but Not N6 Fatty Acids (Intralipid)
Cells
July 14, 2022
Emilie Gueguen, Yasser Morsy, Michael Scharl, Stefanie D. Krämer, Michael Zaugg, Martin Hersberger, Gerhard Rogler, Marcin Wawrzyniak
the
ProLong™ Gold Antifade Mountant with DAPI
(
P36935
-
Thermo Fisher Scientific
). Anti-human F-actin (
ab130935
) and anti-human VE-Cadherin (
ab33168
) (
Abcam
, Cambridge, United Kingdom) antibodies were used in the
(12)
Autophagy, not apoptosis, plays a role in lumen formation of eccrine gland organoids
Chinese Medical Journal
February 5, 2022
Lijie Du, Lei Zhang, Junhong Zhao, Zixiu Chen, Xiang Liu, Manxiu Cao, Lei You, Yonghong Zhang, Xiaobing Fu, Haihong Li
and 3C]. The corresponding antibodies are as follows: Ki67 (Invitrogen SP6,
1:1000
), F-actin (
Abcam
ab130935
,
1:500
),
LC3B
(
CST
3868T
,
1:1000
),
PARP
(
CST
9542
,
1:1000
), cleaved
caspase-3
(
CST
9664
s,
1:1000
),
(13)
Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons
Neural Regeneration Research
January 12, 2022
Qian-Ru Ding, Qian-Ru Cong, Fan-Hui Cong, Fan-Hui Yu, Yu-Hua Ji, Hai-Yan Shi, Hai-Yan Ding
anti-p-TrkB (
MilliporeSigma
, Cat#
ABN1381
, RRID: AB_2721199,
1:500
), mouse anti-F-actin antibody (
Abcam
, Cat#
ab130935
,
1:400
),
mouse
anti-β-Actin antibody (
Abcam
, Cat#
ab8224
, RRID: AB_449644,
1:400
), and rabbit anti-ChAT
(14)
SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathways
Theranostics
January 1, 2022
Ma L, Zou R, Shi W, Zhou N, Chen S, Zhou H, Chen X, Wu Y.
Millipore
), and incubated overnight at 4 °C with corresponding primary antibodies: F-actin (
1:1000
,
Abcam
, #
ab130935
), G-actin (
1:1000
,
Abcam
, #
ab200046
), p-
CaMKII
(
1:1000
,
Abcam
, #
ab124880
), cofilin (
1:1000
,
Abcam
, #
ab54532
),
(15)
Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway
Frontiers in medicine
January 1, 2022
Feng L, Li H, Du Y, Zhang T, Zhu Y, Li Z, Zhao L, Wang X, Wang G, Zhou L,
et al
.
with different primary antibodies: anti-
MERTK
(
Cell Signaling Technology
,
4319S
), anti-F-actin (
Abcam
,
ab130935
), anti-
CCT5
(
Proteintech
,
11603-1-AP
), anti-cofilin (
Abcam
,
ab54532
), anti-phosphorylated cofilin (p-cofilin,
(16)
Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1
Frontiers in Cell and Developmental Biology
March 12, 2021
Li Zhang et al.
binding, the
membrane
was incubated in different primary antibodies:
CFL1
(
CST
#
5175
), F–actin (
Abcam
#
ab130935
), G–actin (
Abcam
#
ab123034
),
GAPDH
(
Abcam
#
ab128915
). All antibodies were used at a
1:1,000
dilution,
(17)
Mechanical stretch promotes hypertrophic scar formation through mechanically activated cation channel Piezo1
Cell Death & Disease
March 1, 2021
Jiahao He, Bin Fang, Shengzhou Shan, Yun Xie, Chuandong Wang, Yifan Zhang, Xiaoling Zhang, Qingfeng Li
samples: anti-Piezo1 (Abcam,
ab128245
,
1:100
), anti-
Ki67
(
CST
, #
9449
,
1:400
), anti-F-actin (
Abcam
,
ab130935
,
1:500
), anti-α-SMA (
CST
, #
48938
,
1:200
), Alexa Fluor 594
goat
anti-rabbit secondary antibody (Jackson
(18)
Nerve growth factor regulates liver cancer cell polarity and motility
Molecular Medicine Reports
February 19, 2021
Haihua Lin, Huanjun Huang, Yuhong Yu, Weibin Chen, Shaomin Zhang, Yuanzhi Zhang
Inc.), vimentin (
1:1,000
; cat. no.
3932
;
Cell Signaling Technology
, Inc.), F‑actin (
1:1,000
; cat. no.
ab130935
;
Abcam
) and β‑actin (
1:1,000
; cat. no.
sc69879
;
Santa Cruz Biotechnology Inc
.) were used for different
(19)
ICAM-1 Activates Platelets and Promotes Endothelial Permeability through VE-Cadherin after Insufficient Radiofrequency Ablation
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
February 1, 2021
Kong J, Yao C, Dong S, Wu S, Xu Y, Li K, Ji L, Shen Q, Zhang Q, Zhan R,
et al
.
antibodies including anti‐VE‐cadherin (
1:200
;
ab33168
, Abcam, London, UK) and anti‐F‐actin (
1:200
;
ab130935
,
Abcam
, London, UK) overnight at 4 °C. Plates were washed and were then incubated with anti‐
mouse
or
(20)
Radiation-induced abscopal reproductive effect is driven by TNF-α/p38 MAPK/Rac1 axis in Sertoli cells
Theranostics
January 1, 2021
Hu S, Zhu L, Song Y, Zhao X, Chen Q, Pan Y, Zhang J, Bai Y, Zhang H, Shao C.
(
ab51129
,
Abcam
), TNF-α (
ab183218
,
Abcam
),
ZO-1
(
ab216880
,
Abcam
),
Occludin
(
33-1500
,
Invitrogen
), F-actin (
ab130935
,
Abcam
),
p38
(
ab170099
,
Abcam
), p-
p38
(
ab195049
,
Abcam
), β-actin (AF0003, Beyotime), α-tubulin (AF0001,
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Reviews
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Anti-F-actin antibody [4E3.adl] from Abcam
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
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