Fig 1: Rbfox1 is expressed in RGCs and ACs(A) In the GCL, all Rbpms-positive cells, RGCs, were also stained for Rbfox1 (yellow arrows point at some Rbpms/Rbfox1-positive cells). Red arrows point at some Rbfox1-positive/Rbpms-negative cells that are most likely displaced ACs. (B) In the INL, virtually all clabindin-positive cells were also Rbfox1-positive cells (yellow arrows). Very few Rbfox1-immunostained cells appeared to be calbindin-negative (red arrows). In the GCL, Rbfox1 is expressed in calbindin-positive displaced ACs (yellow arrows), as well as in calbindin-negative cells, many of which are RGCs (red arrows). (C) Colocalization of Rbfox1 expression with RGCs in whole mount retinas. All Rbpms-positive cells were immunostained with Rbfof1. Rbfox1-positive/Rbpms-negative cells (presumably displaced ACs) are indicated by white arrows. (D) A significant overlap in Rbfox1 and calbindin expression within GCL was observed in whole mount retinas (yellow arrows). Rbfox1-positive/calbindin-negative and calbindin-positive/Rbfox1-negative cells are pointed with red and green arrows, respectively. DAPI; 4',6-diamidino-2-phenylindole; GCL; ganglion cell layer, INL; inner nuclear layer, IPL; inner plexiform layer, ONL; outer nuclear layer, OPL; outer plexiform layer.