Fig 1: Detection of PICK1 and PKC signaling in brain tissues.The brain samples from WT and Tspan7-/- rats were, respectively, homogenized in RIPA lysis buffer, and the total proteins were obtained and analyzed by Western blot using primary antibodies of anti-p-ERK (4370; dilution of 1:1,000; CST), ERK (9102; dilution of 1:1,000; CST), p-PKCe (ab63387; dilution of 1:500; Abcam), PKC; (2683; dilution of 1:500; CST), p-PKCa (9375; dilution of 1:1,000; CST), PKCa (2056; dilution of 1:500; CST), PICK1 (10983-2-AP; dilution of 1:1,000; ProteinTech), NMDAR1 (ab17345; dilution of 1:500; Abcam), and secondary antibodies of HRP-labelled goat anti-mouse IgG, HRP-labelled goat anti-rabbit IgG. Proteins were visualized using a chemiluminescent detection system and analyzed using Quantity One software. (A) Phosphorylated and total ERK, PKCe, PKCa, PICK1, and NMDAR1 proteins in the brain tissues from WT and Tspan7-/- rats were detected with Western blot. (B, C, D, E, F) Phosphorylated and total ERK, PKCe, PKCa, PICK1, and NMDAR1 proteins in the brain tissues from WT and Tspan7-/- rats were quantified with ImageJ. (G) Body weights and birth rates were compared between WT and Tspan7-/- rats at 2, 3, 4, and 8 wk. The results showed that the PICK1, ERK, PKC, and NMDAR1 were not changed obviously in Tspan7-/- rats and suggesting PICK1 or ERK signal could be not the main pathway of Tspan7-/-. Data information: In (B, C, D, E, F), data were analyzed by two-tailed unpaired t tests. Error bars represent SEM; n.s, not significant.Source data are available for this figure.
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