Fig 2: Replication of FLG knockdown LSE findings in AE skin. Representative immunofluorescence images of normal (FLG wild-type), uninvolved (UN AE), and involved (INV AE) AE skin (n = 3). Green staining depicts FLG, PPIA, KLK7, or CFL1, as indicated. Hoechst 33342 nuclear counterstaining is shown in red (-50 µm). Dashed lines represent the epidermal/dermal boundary. A, Corresponding quantification values are outlined in red on the bottom scatter plots. B-E, Scatter plots of the corresponding protein florescence intensities expressed as function of unit area and on a log10 scale (means ± 95% CIs). All subjects are FLG wild-type unless indicated in orange (FLG heterozygous) or green (FLG homozygous/compound heterozygous).