Fig 2: a Comparing expression levels of different DLC1 transcription variants in normal meningeal sections. b–e: Comparing expression levels of particular DLC1 isoforms in normal meninges and meningiomas. Horizontal lines represent the mean value

Fig 3: a Examples of immunohistochemical staining of DLC1-v1/-v3 protein expression in meningiomas. Magnification ×400. b Comparison of DNA methylation levels in meningiomas with high/moderate DLC1 expression and meningiomas with none/poor DLC1 expression. c Comparing the relative expression levels of DLC1v1 and DLC1v3 measured by 220511_s_at probeset (HG U133A Array, Affymetrix), GSE43290 dataset. Horizontal lines represent the mean value

Fig 4: a The expression of DLC1 transcription variants in KT21 meningioma cells, qRT-PCR results. b Silencing of DLC1-v1 expression in KT21 cells through transfection with plasmid vector containing specific shRNA cassette as compared with cells transfected with control non-effective vector. Cells’ transfection efficacy assessed with flow cytometry, DLC1-v1 mRNA and protein level comparison between KT21 cells transfected with DCL1 specific (sh DLC1-v1) and non-specific shRNA vectors sh control. c Active RHO-GTPases pull-down assay in KT21 cells transfected with shRNA targeting DLC1 and control cells. RHO-GTPases were detected with antibody specific to RhoA, RhoB and RhoC. d Result of MTT test performed in KT21 cells transfected with shRNA targeting DLC1 and control cells. e The results of woundhealing assay performed in KT21 cells transfected with shRNA targeting DLC1 and control cells. The examplary fields marked for the measurement of the migration distance. The comparison of migration distance of shDLC1-1 and sh-control KT21 cells.