Fig 2: Correlation between mRNA and protein expression in two cluster of gastric cancer samplesA. YWHAE and CDC25B mRNA expression in the cluster 1. B. YWHAE and MYC mRNA expression in the cluster 1. C. CDC25B and MYC mRNA expression in the cluster 1. D. YWHAE and CDC25B protein expression in the cluster 1. E. YWHAE and MYC protein expression in the cluster 1. F. CDC25B and MYC protein expression in the cluster 1. G. YWHAE and CDC25B mRNA expression in the cluster 2. H. YWHAE and MYC mRNA expression in the cluster 2. I. CDC25B and MYC mRNA expression in the cluster 2. J. YWHAE and CDC25B protein expression in the cluster 2. K. YWHAE and MYC protein expression in the cluster 2. L. CDC25B and MYC protein expression in the cluster 2. Correlation coefficients and p-values of Spearman correlation test are shown. *p<0.05, significant correlation by S Spearman correlation test.

Fig 3: YWHAE, CDC25B and MYC immunoreactivity in gastric tissue samplesA. Non-neoplastic gastric mucosa showing cytoplasmic YWHAE staining. B. intestinal-type gastric cancer cells without YWHAE immunoreactivity. C. Non-neoplastic gastric tissue without CDC25B immunoreactivity. D. Diffuse-type gastric cancer presenting nuclear and cytoplasmic CDC25B immunoreactivity. E. Gastric mucosa without MYC immunoreactivity. F. Intestinal-type gastric cancer presenting nuclear immunoreactivity of MYC.

Fig 4: Effect of gene silencing in gene and protein expression in gastric cancer cell linesA. YWHAE silencing induced MYC and CDC25B increased expression in GC cell lines. B. GC cells with (+) or without (-) YWHAE silencing, equal amounts of whole cell extracts were analyzed by western blot with the indicated antibodies. C. CDCD25B silencing did not alter MYC and YHWAE expression in GC cell lines. D. GC cells with (+) or without (-) CDC25B silencing; Equal amounts of whole cell extracts were analyzed by western blot with the indicated antibodies. E. MYC silencing induced the reduction of CDC25B expression and increasing of YHWAE expression in GC cell lines. F. GC cells with (+) or without (-) MYC silencing; equal amounts of whole cell extracts were analyzed by western blot with the indicated antibodies. siRNA control-transfected cells were used as a calibrator. Values of median and IQR are shown.

Fig 5: Correlation between protein expression in YWHAE-silenced AGP01, ACP02 and ACP03 gastric cancer cell linesA. YWHAE and MYC. B. YWHAE and CDC25B. C. MYC and CDC25B. Correlation coefficients and p-values of Spearman correlation test are shown. *p<0.05, significant correlation by Spearman correlation test.