Fig 2: Mechanism of OLA1 increases the resistance of paclitaxel in breast cancer. (A) IPA analysis between OLA1 and γ-tubulin in cell cycle regulation. (B) OLA1 and γ-tubulin levels in indicated cells. (C) Mechanism of OLA1 regulates the resistance in different breast cancer cells.

Fig 3: OLA1 is upregulated in breast cancer. (A) The average expression level of OLA1 in patient’s breast cancer tissue was higher than adjacent tissues in TCGA and GTEx breast cancer dataset. (B, C) Kaplan-Meier overall survival and relapse free survival curves for patients with breast cancer stratified by high and low expression of OLA1.

Fig 4: Knockdown of OLA1 suppress the chemoresistance and enhanced chemo-sensitivity of acquired drug resistance cell in breast cancer. (A, B) were mRNA and protein levels of OLA1 with or without siOLA1 in MCF-7-PTR. (C, D) Depletion of OLA1 enhances the PTX or 5-Fu sensitivity of MCF-7-PTR cells by specific siRNA respectively. (*P < 0.05, **P < 0.01, ***P < 0.001, Unpaired Student’s t-test.) (E) Annexin V-FITC and PI staining of the indicated cells treated with PTX (20 µM) for 24 h (apoptosis rate is the sum of early and late apoptosis rates). (*P< 0.05, ***P < 0.001, Unpaired Student’s t-test.) (F) Western blotting analysis of cleaved caspase3, Bax, and Bcl2 in the indicated cells; GAPDH was used as a loading control. (G) Cell proliferation curve was drawn in the indicated cells. ns, no significance.

Fig 5: OLA1 induces EMT phenotype by TGFß/Smad pathway in breast cancer. TGFß/Smad signaling marker mRNA level was confirmed in MCF-7-PTR (A), and MDA-MB-231-shOLA1 (B) compared with their Control. Wnt signaling marker mRNA level was confirmed in MCF-7-PTR (C), and MDA-MB-231-shOLA1 (D) compared with their Control. (E) Protein level of Smad3, p-Smad3 and Smad 4 in MCF-7-PTR was calculated comparing with MCF-7. (F) Protein level of Smad3, p-Smad3 and Smad 4 in MDA-MB-231-shOLA1 was calculated comparing with MDA-MB-231. (G) mRNA level Pearson Correlation analysis between OLA1 and TGFB1. Left: Relationship in GTEx Breast mammary tissue and TCGA BRCA normal data. Right: Relationship TCGA BRCA tumor data. (H) mRNA level Pearson Correlation analysis between OLA1 and Smad3. Left: Relationship in GTEx Breast mammary tissue and TCGA BRCA normal data. Right: Relationship TCGA BRCA tumor data. (I) mRNA level Pearson Correlation analysis between OLA1 and Smad4. Left: Relationship in GTEx Breast mammary tissue and TCGA BRCA normal data. Right: Relationship TCGA BRCA tumor data. (**P < 0.01, ***P < 0.001, Student’s t-test). ns, no significance.