Fig 1: PCR and immunofluorescence analysis of ObSSCs. (A) Expression of germ cell markers by RT-PCR of total RNA from ObSSCs and adult gonads with primers for dazl, dnd, vasa, and gfra1. (B) Expression of stem cell marker by RT-PCR with primers for nanog. (C) Expression of Sertoli cell marker by RT-PCR with primers for dmrt1 (asterisk). (D) Actin expression was determined for calibration. (B–G) Immunofluorescence of Vasa (green) and PCNA (red) in ObSSCs. (Bars = 20 µm).