Fig 2: Distributions of FLI1 H-scores for different types of gastric tissueBean plots showing the distribution of FLI1 H-scores for normal, intestinal metaplasia (IM), low-grade dysplasia (LGD), high-grade dysplasia (HGD), and gastric adenocarcinoma (GC), with mean H-scores (represented by the largest line in each bean plot) of 187.9 (SEM = 3.25), 193.7 (SEM = 2.37), 172.3 (SEM = 15.2), 140.6 (SEM = 14.8), and 108.2 (SEM = 14.6), respectively. A P value <0.05 was considered statistically significant. Significant differences in FLI1 H-scores were detected for normal vs GC (P = 1.2×10-5), IM vs GC (P = 1.4×10-6), and LGD vs GC (P = 0.006).

Fig 3: Representative nuclear FLI1 IHC staining in normal, IM and gastric adenocarcinoma tissuesAntral glands (A), oxyntic glands (B), IM (C), representative tumor with high FLI1 H-score of 199 (D–F), representative tumor with low FLI1 H-score of 88 (G–I). E, F, H and I show tumor samples with nuclear FLI1 IHC staining, at low (E and H) and high magnification (F and I). (I) Arrows point to tumor nuclei with little to no FLI1 positivity. Arrowhead points to stromal cell nucleus with strong FLI1 positivity, serving as internal positive control. A-D and G are H&E stains. Low power H&E (J) of stomach with high-grade dysplasia (K) and invasive adenocarcinoma (L). High power view of K and L inset are shown as K* and L*. Panel M shows FLI1 IHC of the case represented in panel J. High-grade dysplasia with preserved nuclear FLI1 expression is shown in the inset N and as high power view in N*. Invasive adenocarcinoma with loss of nuclear FLI1 expression is shown in the inset O and as high power view in O*, where the arrows indicate the nuclei of invasive adenocarcinoma. Original magnification 200×.

Fig 4: Survival plots of patients with optimal cutoffs for high and low FLI1 H-scoresSurvival plots displaying survival probability (y-axis) vs time in days (x-axis). (A) Survival plot for all patients analyzed in the TMAs with FLI1 H-score cutoff of 100 to separate high and low FLI1-expressing tumors. (B) Survival plot for all patients with well and moderately differentiated tumors, using a FLI1 H-score cutoff of 100 to separate high and low FLI1-expressing tumors. (C) Survival plot for all patients with poorly differentiated tumors, using a FLI1 H-score cutoff of 110.5 to separate high and low FLI1-expressing tumors. The Cox proportional hazards (PH) model and the Mantel-Haenszel log-rank P values are displayed. A P value <0.05 was considered statistically significant.