Fig 1: Evidence for systemic activation of SNS at 3 ?h following stroke. Noradrenaline (NA) is synthesized from dopamine by the dopamine ß-hydroxylase (DBH) enzyme (A). Naïve mice and mice that underwent pMCAO or sham surgery and were culled and plasma was collected via cardiac puncture at 15 ?min (B), 30 ?min (C), 60 ?min (D) and 3 ?h (E) post-surgery to assess the levels of plasma NA. Data are represented as mean ?± ?SEM, n ?= ?3 mice per group in 15 ?min, 30 ?min and 60 ?min timepoints, and n ?= ?5 for 3 ?h timepoints, p ?= ?0.08, t-tests. (F) The protein levels of UCP-1 in the iBAT of sham-operated and post-stroke mice were assessed via Western blots at 3 ?h and 24 ?h. Data are represented as mean ?± ?SEM, n ?= ?4 mice per group. *p ?< ?0.05, t-tests.