Fig 1: 14-3-3? negatively regulates mitochondrial biogenesis in RR cells. (A) Transmission electron micrographs of non-irradiated and RR cells of SF268 and U87MG after 14-3-3? knockdown (14-3-3 zeta KD) showing mitochondria (yellow arrows). Cells with scrambled shRNA (Src) are the control. Scale bar 1 µm. (B and C) Dot plot quantifies mitochondrial number per cell of SF268 and U87MG in different conditions, as mentioned. Each data point represents the mean of at least 10 cells from two independent experiments. * denotes P = 0.05, ** denotes P = 0.01. (D) Graph shows fold change of mitochondrial DNA content in non-irradiated and RR cells of SF268 following 14-3-3? knockdown. Data represent mean ± SD of three independent experiments. * denotes P = 0.05.