Fig 1: SIRT1 inhibits the transcription activation of YAP by suppressing the acetylation of C/EBPß K39 and histone 3 (H3) K9 and K27 in HFs(A) RT-qPCR results showing the mRNA levels of CEBPB, SIRT1, and YAP in HFs transfected with C/EBPß overexpression plasmids (pcDNA3.1(+)-C/EBPß) or/and SIRT1 overexpression plasmids (pEX-3-SIRT1).(B and C) Western blot results show the protein levels of C/EBPß, SIRT1, and YAP in different groups.(D) Dual-luciferase assay was used to detect the luciferase activity of the reporter gene vectors containing wild-type YAP promoter (pGL3-YAP-WT) in different groups.(E) ChIP-qPCR to detect the effect of SIRT1 overexpression on the enrichment of C/EBPß at the YAP promoter.(F–H) The effect of SIRT1 silencing on C/EBPß acetylation level (F, G) and YAP expression (F, H) in HFs transfected with C/EBPß overexpression plasmid (pcDNA3.1(+)-C/EBPß) or C/EBPß K39 acetylation-defective plasmid (pcDNA3.1(+)-C/EBPß K39R) was evaluated by IP combined with Western blot.(I and J) ChIP-qPCR assay to detect the effect of SIRT1 overexpression on the acetylation levels of H3K9 and H3K27 at the C/EBPß binding region of the YAP promoter. Data are represented as mean ± SD. ns, p>0.05, *p < 0.05, **p < 0.01, and ***p < 0.001. The differences were analyzed by One-way ANOVA with Tukey’s multiple-comparison tests.