Fig 1: The miR-23a cluster does not regulate 14-3-3?, or other 14-3-3 isotypes.(A) and (B) The effect of the miR-23a cluster on (A) 14-3-3? and (B) 14-3-3? protein expression was measured via Western blot. The top panels are representative blots from one experiment, and the bottom panels are graphs of normalized protein expression from 3 independent experiments (±SEM), determined by densitometry analysis (p<0.05*). (C) The schematics show predicted miR-27a and miR-24 binding sites in the CDS and 3'UTR of 14-3-3? (top panel). HEK293T cells were co-transfected with 14-3-3?L-157, 14-3-3?L-627, or 14-3-3?L-1075 (500 ng, white bars) alone and with 25 nM of miR-23a (light grey bars) or miR-24 (dark grey bars); HEK293 cells were also co-transfected with 14-3-3?L-1266 (500 ng, white bars) alone and with 25 nM of miR-23a (light grey bars) or miR-27a (black bars) (bottom panel). Normalized mean Luc expression (±SEM, n=3) is represented on the graphs.