Fig 1: Genes required for modification of the anticodon loop of Phe-GAA and Lys-UUU tRNAs are poorly expressed in oligodendrocytes.a Heatmap of the expression levels (FPKM) of the factors involved in the modification pathways of Phe-GAA ACL (top) and Lys-UUU ACL (bottom), data from111 for the whole cortex (WC), OPCs, Newly Formed Oligodendrocytes (NFO), Myelinating Oligodendrocytes (MO) and astrocytes (Astro) purified from mouse brain. The numbers are the biological replicates numbers. b Expression levels (FPKM) from RNA-Seq data from (a) for examples of genes differentially expressed between OPCs and Myelinating oligodendrocytes (MO) (p < 0.05), in the whole cortex (n = 3 biological replicates), OPC (n = 2 biological replicates), NFO (n = 2 biological replicates), MO (n = 2 biological replicates) and Astro (n = 2 biological replicates). c Expression levels (FPKM) from RNA-Seq data from OPCs and OPCs differentiated to oligodendrocytes (OL), for the factors involved in the modification pathways of Phe-GAA ACL and Lys-UUU ACL, expressed as a ratio of FPKM OPC/OL. These values are the average from 5 biological replicates for OPCs and four biological replicates for oligodendrocytes. DESeq2 analysis: adjusted p value < 0.05 for Thada, Urm1, Elp1, Elp3, Elp6 and Kti12 (adjusted p value: Benjamini–Hochberg correction). d Western blot analyses of TYW3, ELP1 and ELP3 in OPCs, oligodendrocytes (OLs) and astrocytes (Astro) (OLs and Astro differentiated from OPCs). The numbers represent two biological replicates. ?eta-actin is used as a loading control. The signals from the Western blots were quantified and expressed relative to the levels in OPCs.