Fig 1: Immunostaining analysis of astrocytes in astrocyte-specific Sirt1-knockout and WT EAE mice.Spinal cords from AAV-sgSirt1– and AAV-sgScram–injected mice with EAE were costained for GFAP and CHI3L1 (A), SRXN1 (C), and TRAIL (E). Scale bars: 100 µm and 100 µm (enlarged insets). Analysis of costaining results for CHI3L1 (B), SRXN1 (D), and TRAIL (F). n = 5 mice per group, unpaired, 2-tailed t test. All results are expressed as the mean ± SD. ****P < 0.0001, by unpaired, 2-tailed t test. Data from 1 representative experiment of 2 are shown.