Fig 2: (a) Flow cytometry was used for gating HLA-DR-CD11b+CD15+ CD33+ LDNs. Data were shown as proportions of LDNs in whole blood cells. (b) Variation of LDN ratios and counts during different GBS courses. (c) Association between low-density neutrophils (LDNs) and disease severity. Pearson's correlation was used to analyze the relevance of LDN counts (upper) and LDN ratios (lower) to the Medical Research Council sum score (*** p < .001)

Fig 3: Fcmr inhibits skin DC migration, phagocytosis, and maturation. a Left: Quantification of total inguinal LN DCs in Fcmr+/+ and Fcmr-/- mice at 24 h after ventral skin painting with FITC-dibutylthalate. Right: Representative flow cytometry plot showing the gating strategy. Data are representative of n = 5 Fcmr+/+ and 6 Fcmr-/- mice. b Representative flow cytometry plots illustrating the gating strategy to identify the CD11b+ and CD11b- subpopulations of dermal DCs (dDC) as well as Langerhans cells (LC) within the total DC population in the inguinal LN of the Fcmr+/+ and Fcmr-/- mice painted with FITC-dibutylthalate in (a). c Quantification of the flow cytometry data collected on the two dermal DC and LC populations in (b). Each data point represents an individual mouse (n = 5 Fcmr+/+ and 6 Fcmr-/- mice). d, e Left: Representative histograms showing FITC-negative and -positive subsets of the d dDC and e LC populations in the inguinal LN of the Fcmr+/+ and Fcmr-/- mice in (a–c). Right: Quantification of the number of FITC-positive d dDC and e LC in the left panels. f, g Left: Representative histograms depicting cell surface levels of CD80 and CD86 on f dDC and g LC in the inguinal LN of the Fcmr+/+ and Fcmr-/- mice in (a–e). Right: GMFI quantification of CD80 and CD86 expression levels on the f dDC and g LC in the left panel. Data are represented as mean ± SEM (t test; *p < 0.05; **p < 0.01; ***p < 0.001)

Fig 4: Dex promotes neutrophil and macrophage recruitment in acute ulcerative colitis. The relative proportions of macrophages (CD11b+F4/80+), neutrophils (CD11b+Ly6G+), dendritic cells (DCs) (CD11c+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+) and B cells (CD19+) in single-cell suspensions derived from mice treated with 3% dextran sulfate sodium (DSS) for 5 days in combination with dexamethasone (Dex) or vehicle (phosphate buffer saline; PBS), with daily intraperitoneal (i.p.) injection. (A,C) Single-cell suspensions of colonic tissue from mice were isolated. Representative flow cytometry figures are shown (A), and the data are summarized (C). The blood cells of the mice were analyzed. The CD11b+CD115+ cells were used as a marker of monocytes. A representative flow cytometry figure is shown (B, upper), and the data are summarized (D). The bone marrow cells of mice were analyzed, and a representative flow cytometry figure is shown (B; lower); the data are summarized (E). Representative results are based on one of three or four independent experiments performed with similar results. The data are presented as the mean ± SD (n = 4–8 mice per group). Statistical significance was measured by one-way ANOVA. ***P< 0.001, compared with the indicated groups.

Fig 5: Suppressive neutrophils in LCMV clone 13 infection have an oxidative metabolic phenotype(A–D) Representative flow cytometry plots and histograms depicting (A) MitoTracker Green, (B) tetramethylrhodamine ethyl ester (TMRE), (C) 2-NDBG, and (D) BODIPY C-16 staining in Ly6C+ Ly6G- monocytes and Ly6C+ Ly6G+ neutrophils from 14 d.p.i. with chronic LCMV clone 13. Cells were gated on CD11b+ F4/80- single cells. Data are shown as mean ± SEM. *p < 0.05 by paired t test. n = 5 for each condition. Data were collected from 2 independent experiments.