Anti-IL-10 antibody [JES5-2A5] ab33471 from Abcam

Product Specs
ItemAnti-IL-10 antibody [JES5-2A5]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab33471
Domain/Region/Terminus5-2
Antibody TypePrimary
Quantity100 UG
ClonalityMonoclonal
IsotypeIgG1
HostRat
CloneJES5-2A5
Conjugate/TagUnconjugated
FormatPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NameIL10
ReactivityMouse
TargetIL-10

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Lonicera japonica Thunb extract ameliorates lipopolysaccharide-induced acute lung injury associated with luteolin-mediated suppression of NF-κB signaling pathwayJ Inflamm (Lond)December 19, 2023Qinyao Jia, Jing Wen, Qi Yang, Shengming Liu, Jianwu Zhang, Tao Wang, Yao ChengAbcam), IL-1β (31 kDa, ab283818, 1:1000, Abcam), TNF-α (26 kDa, ab6671, 1:1000, Abcam), IL-10 (20 kDa, ab33471, 1:1000, Abcam), medullary differentiation protein 88 (Myd88, 33 kDa, ab133739, 1:5000, Abcam), Phospho-nuclear

(2) Low-dose LPS alleviates early brain injury after SAH by modulating microglial M1/M2 polarization via USP19/FOXO1/IL-10/IL-10R1 signalingRedox BiologyOctober 1, 2023Weihua Tao, Guibo Zhang, Chengyuan Liu, Lide Jin, Xuehua Li, Shuaifeng Yang(1:1000, ab22604), anti-USP19 (1:1000, ab93159), anti-FOXO1 (1:1000, ab52857), anti-IL-10 (1:1000, ab33471), anti-IL-10R1 (1:1000, ab89883), anti-MMP9 (1:1000, ab38898) and anti-β-actin antibodies (1:2000, ab8226,

(3) Amitriptyline-Based Biodegradable PEG-PLGA Self-Assembled Nanoparticles Accelerate Cutaneous Wound Healing in Diabetic RatsPharmaceuticsAugust 26, 2022Hani Z. Asfour, Nabil A. Alhakamy, Osama A. A. Ahmed, Usama A. Fahmy, Mohamed A. El-moselhy, Waleed Y. Rizg, Adel F. Alghaith, Basma G. Eid, Ashraf B. Abdel-NaimIL-10 anti-TGF-β1, anti-VEGF-A, anti-PDGF-B, collagen IV and CD31 overnight (Cat. No. ab9324, ab220210, ab33471, ab215715, ab1316, ab23914, ab6586 and ab182981, respectively, ABCAM, Cambridge, UK), all at 1 μg/mL.

(4) IL-10 Dysregulation Underlies Chemokine Insufficiency, Delayed Macrophage Response, and Impaired Healing in Diabetic WoundsThe Journal of investigative dermatologyMarch 1, 2022Roy R, Zayas J, Mohamed MF, Aboonabi A, Delgado K, Wallace J, Bayat M, Kuzel TM, Reiser J, Shafikhani SH.No. 52746 Anti-MyD88 antibody,Abcam,Cat. No. ab135693 Anti-IL-10 antibody [JES5-2A5],Abcam,Cat. No. ab33471 "InVivoMAb anti-mouse IL-10R (CD210), clone: 1B1.3A",InVivoMab Antibodies,Cat. No. BE0050 Recombinant

(5) Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3Cellular and molecular life sciences : CMLSFebruary 19, 2022Wu Q, Sun S, Wei L, Liu M, Liu H, Liu T, Zhou Y, Jia Q, Wang D, Yang Z, et al.antibodies were used: anti-Twist1 [#49254 (1:2000), Abcam, Cambridge, United Kingdom], anti-IL-10 [ab33471 (1:200), Abcam, United Kingdom], anti-YM1 [#60130 (1:1000), Stemcell Technologies Inc, Vancouver, Canada],

(6) Adipose Mesenchymal Stromal Cell-Derived Exosomes Prevent Testicular Torsion Injury via Activating PI3K/AKT and MAPK/ERK1/2 PathwaysOxidative medicine and cellular longevityJanuary 1, 2022Liu H, Shi M, Li X, Lu W, Zhang M, Zhang T, Wu Y, Zhang Z, Cui Q, Yang S, et al.anti-CCR7 (ab32527; Abcam), anti-CD163 (ab182422; Abcam), anti-IL-6 (TA500067S; Origene), and anti-IL-10 (ab33471; Abcam), followed by incubation with secondary antibodies (SA00013; ProteinTech, Chicago, IL, USA).

(7) Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Promote Peritoneal Healing by Activating MAPK-ERK1/2 and PI3K-Akt to Alleviate Postoperative Abdominal AdhesionStem cells internationalJanuary 1, 2022Shi M, Liu H, Zhang T, Zhang M, Tang X, Zhang Z, Lu W, Yang S, Jiang Z, Cui Q, et al.(CCR7; rabbit monoclonal; 1 : 200; ab32527; Abcam), interleukin- (IL-) 10 (rabbit monoclonal; 1 : 100; ab33471; Abcam), IL-6 (mouse monoclonal; 1 : 100; TA500067; Origene), COX-2 (rabbit monoclonal; 1 : 100; ab179800

(8) Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitisStem Cell Research & TherapyDecember 1, 2021Ning Li, Yichi Zhang, Narayan Nepal, Guoqing Li, Ningning Yang, Haoyuan Chen, Qiuchi Lin, Xuechun Ji, Sijia Zhang, Shizhu Jin(Santa Cruz Biotechnology, sc52746, 1:500), TGF-β (Cell Signaling Technology, #3711, 1:500), IL-10 (Abcam, ab33471, 1:1000), IFN-γ (R&D Systems, MAB585, 1:1000), HGF (Abcam, ab83760, 1:500) Results Verification of

(9) Inhibition of vascular adhesion protein 1 protects dopamine neurons from the effects of acute inflammation and restores habit learning in the striatumJournal of NeuroinflammationDecember 1, 2021Serena Becchi, Alberto Buson, Bernard W. Balleine(1:1000),AB7260 Rabbit anti-interleukin 1 beta,Santa Cruz,SC-7884 Rat anti-interleukin 10,Abcam (1:500),AB33471 Goat anti-ionized calcium binding adapter molecule,Abcam (1:2000),AB5076 Rabbit anti-ionized calcium

(10) Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3Stem cell research & therapyJune 10, 2021Liu H, Zhang M, Shi M, Zhang T, Lu W, Yang S, Cui Q, Li Z.ab182422; Abcam), anti-IL-6 (monoclonal; 1:100; TA500067S; Origene), and anti-IL-10 (monoclonal; 1:100; ab33471; Abcam). The sections were then incubated with secondary antibodies (1:200; SA00013; Proteintech, Rosemount, IL,

(11) Etomidate alleviates cardiac dysfunction, fibrosis and oxidative stress in rats with myocardial ischemic reperfusion injuryAnnals of Translational MedicineNovember 26, 2020Dili Xie, Min Li, Kang Yu, Hua Lu, Yong Chenantibodies used were: TGF-β (ab31013), α-SMA (ab32575), fibronectin (ab32419), iNOS (ab213987), IL-10 (ab33471), GAPDH (ab181602) and all secondary antibodies were from Abcam (Cambridge, UK). Bands were analyzed

(12) Inhibition of EZH2 (Enhancer of Zeste Homolog 2) Attenuates Neuroinflammation via H3k27me3/SOCS3/TRAF6/NF-κB (Trimethylation of Histone 3 Lysine 27/Suppressor of Cytokine Signaling 3/Tumor Necrosis Factor Receptor Family 6/Nuclear Factor-κB) in a Rat Model of Subarachnoid HemorrhageStrokeNovember 1, 2020Yujie Luo, Yuanjian Fang, Ruiqing Kang, Cameron Lenahan, Marcin Gamdzyk, Zeyu Zhang, Takeshi Okada, Jiping Tang, Sheng Chen, John H. Zhang(1:1000, ab6672, Abcam, United States), TNF-α (1:1000, ab1793, Abcam, United States), and IL-10 (1:1000, ab33471, Abcam, United States) overnight at 4°C. On the following day, secondary antibodies were incubated at

(13) Aging-Related Phenotypic Conversion of Medullary Microglia Enhances Intraoral Incisional Pain SensitivityInternational Journal of Molecular SciencesOctober 23, 2020Daisuke Ikutame, Kentaro Urata, Tatsuki Oto, Shintaro Fujiwara, Toshimitsu Iinuma, Ikuko Shibuta, Yoshinori Hayashi, Suzuro Hitomi, Koichi Iwata, Masamichi ShinodaCD163 antibody (1:1000, ab182422; Abcam, Cambridge, UK) and a rat monoclonal IL-10 antibody (1:300, ab33471; Abcam, Cambridge, UK) to identify IL-10 positive M2 microglia. Following this primary incubation, the

(14) Ribonuclease T2 from Aspergillus fumigatus promotes T helper type 2 responses through M2 polarization of macrophagesInternational journal of molecular medicineAugust 1, 2020Chen JJ, He YS, Zhong XJ, Cai ZL, Lyu YS, Zhao ZF, Ji K.Cell Signaling Technology Inc.), JNK (cat. no. 9252; Cell Signaling Technology Inc.), IL-10 (cat. no. ab33471; Abcam), induced nitric oxide synthase (iNOS; cat. no. ab213987; Abcam), ARG1 (cat. no. ab124917; Abcam),

(15) Spondias mombin L. attenuates ventricular remodelling after myocardial infarction associated with oxidative stress and inflammatory modulationJournal of Cellular and Molecular MedicineJuly 1, 2020Bruna Letícia Buzati Pereira et al.Santa Cruz Biotechnology, Inc, 1:200); Keap1 (Abcam, ab 66620, 1:2000); IL‐10 (rat monoclonal IgG, ab33471; Abcam, 1:2000); INF‐γ (mouse monoclonal IgG1, ab133566; Abcam, 1:2500); type I collagen (rabbit polyclonal

(16) Puerarin improves graft bone defect through microRNA‑155‑3p‑mediated p53/TNF‑α/STAT1 signaling pathwayInternational Journal of Molecular MedicineMay 6, 2020Yang Zhou, Hongyu Lian, Kexin Liu, Deli Wang, Xuelian Xiu, Zhang Sun(ab199081, Abcam), IL-6 (ab100712, Abcam), TGF-β1 (ab118557, Abcam), IL-2 (ab223588, Abcam), IL-10 (ab33471, Abcam), ALT (ab234579, Abcam), AST (ab263882, Abcam), γ-GT (ab134640, Abcam), ALP (ab256583, Abcam),

(17) Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised micePLoS pathogensMay 1, 2020Simanjuntak Y, Ko HY, Lee YL, Yu GY, Lin YL.57], MCP-1 (Invitrogen, 41–0900), TNF-α (Abcam, ab6671), IL-6 (Invitrogen, mp5-20f3), IL-10 (Abcam, ab33471), VE-cadherin (Abcam, ab33168), HIF-1α (Novus Biologicals, nb100479) and cleaved caspase-3 (Cell Signaling,

(18) Exosome Treatment Enhances Anti-Inflammatory M2 Macrophages and Reduces Inflammation-Induced Pyroptosis in Doxorubicin-Induced CardiomyopathyCellsOctober 9, 2019Dinender K. Singla, Taylor A. Johnson, Zahra Tavakoli Darganimarker, CD206 (1:50; Abcam; cat# 4693) and for anti-inflammatory cytokine, IL-10 (1:50; Abcam; cat# ab33471) Cells 2019, 8, 1224 4 of 21 overnight at 4 ◦ C. Following primary incubation, sections were incubated

(19) Regeneration-associated cell transplantation contributes to tissue recovery in mice with acute ischemic strokePloS oneJanuary 1, 2019Nakayama T, Nagata E, Masuda H, Asahara T, Takizawa S.

(20) Relationship of Inflammatory Cytokines From M1-Type Microglia/Macrophages at the Injured Site and Lumbar Enlargement With Neuropathic Pain After Spinal Cord Injury in the CCL21 Knockout (plt) MouseFrontiers in cellular neuroscienceJanuary 1, 2019Honjoh K, Nakajima H, Hirai T, Watanabe S, Matsumine A.(ab6671, dilution 1:500), IFN-γ (ab133566, dilution 1:1,000), IL-4 (ab11524, dilution 1:500), and IL-10 (ab33471, dilution 1:500) in blocking solution. After washing three times in 0.1 M PBS, the membranes were immersed

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2/2/2017Anti-IL10 Antibody Anup KumarI am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Anti-IL-10 antibody [JES5-2A5] from Abcam

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Anti-IL-10 antibody [JES5-2A5] from Abcam

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