Anti-HCN4 antibody [SHG 1E5] ab32675 from Abcam

Product Specs
ItemAnti-HCN4 antibody [SHG 1E5]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab32675
Antibody TypePrimary
IsotypeIgG1
HostRat
CloneSHG 1E5
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity100 UL
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaNeuroscience
Gene NameHcn4
ReactivityHuman, Rat
TargetHCN4

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophreniaiScienceMay 19, 2023Hsu YH, Pintacuda G, Liu R, Nacu E, Kim A, Tsafou K, Petrossian N, Crotty W, Suh JM, Riseman J, et al.ab133265;RRID: AB_11158532 HCN1,Proteintech Group,Cat# 55222-1-AP;RRID: AB_11182929 HCN4,Abcam,Cat# ab32675;RRID: AB_732770 HNRNPA2B1,Abcam,Cat# ab6102;RRID: AB_305293 IGF2BP1,Proteintech Group,Cat# 22803-1-AP;RRID:

(2) Loss of function of the nuclear envelope protein LEMD2 causes DNA damage-dependent cardiomyopathyThe Journal of clinical investigationNovember 15, 2022Caravia XM, Ramirez-Martinez A, Gan P, Wang F, McAnally JR, Xu L, Bassel-Duby R, Liu N, Olson EN.MA5-12960, clone 13-11; 1:100), Ki67 (Thermo Fisher Scientific, PA5-19462, 1:200), and HCN4 (Abcam, ab32675, clone SHG 1E5; 1:50). Sections were subsequently washed with 0.01% Triton X-100 in PBS 3 times and

(3) RA signaling pathway combined with Wnt signaling pathway regulates human-induced pluripotent stem cells (hiPSCs) differentiation to sinus node-like cellsStem cell research & therapyJuly 18, 2022Yin L, Wang FY, Zhang W, Wang X, Tang YH, Wang T, Chen YT, Huang CX.were transferred onto a nitrocellulose membrane, and incubated with primary antibodies against HCN4 (ab32675; Abcam, Cambridge, MA, USA), TBX18(ab115262; Abcam, Cambridge, MA, USA), ISL-1(abs132916; Abcam, Cambridge,

(4) Deciphering cellular signals in adult mouse sinoatrial node cellsiScienceJanuary 21, 2022Reddy GR, Ren L, Thai PN, Caldwell JL, Zaccolo M, Bossuyt J, Ripplinger CM, Xiang YK, Nieves-Cintrón M, Chiamvimonvat N, et al.antibodies were used to incubate cells overnight at 4°C: (1) monoclonal rat anti-HCN4 (1:300 dilution; Abcam ab32675) and (2) polyclonal goat anti-ACVI (1:100 dilution; Santa Cruz sc68138). Cells were washed with PBS

(5) Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic DysfunctionsFrontiers in Cardiovascular MedicineNovember 26, 2021Farideh Amirrad, Rajasekharreddy Pala, Kiumars Shamloo, Brian S. Muntean, Surya M. Naulicells in the atrioventricular node, tissues were incubated with rat anti-HCN4 antibody (1:1,000; Cat# ab32675; Abcam, Inc.) overnight at 4°C in a humidified chamber followed with AlexaFluor-488 goat anti-rat fluorescence

(6) A novel transgenic Cre allele to label mouse cardiac conduction systemDevelopmental BiologyOctober 1, 2021Peter C. Kahr, Ge Tao, Zachary A. Kadow, Matthew C. Hill, Min Zhang, Shuang Li, James F. Martinimaged with a Nikon Eclipse upright microscope. For immunofluorescent staining, antibodies against Hcn4 (ab32675, Abcam, Cambridge, UK), cardiac Troponin T (cTnT) (ms-295, Thermo Scientific, Waltham, MA, US), Connexin

(7) PDGFRα-Signaling Is Dispensable for the Development of the Sinoatrial Node After Its Fate CommitmentFrontiers in Cell and Developmental BiologyJune 10, 2021Xi Zheng, Fengjiao Wang, Xiaoxiao Hu, Hua Li, Zhen Guan, Yanding Zhang, Xuefeng HuInstitutes of Health). Antibodies used for this study include anti-Pdgfrα (R&D, AF1062), anti-Hcn4 (Abcam, ab32675), anti-Nkx2.5 (Santa Cruz, sc8697), and antiGFP (Abcam, ab13970). 3D Reconstruction Images of consecutive

(8) Synergistic effects of ivabradine and metformin in the treatment of concomitant chronic heart failure and diabetes mellitus by regulating the activity of the H19/miR-423-5p/HCN4 axisArchives of Medical ScienceMarch 26, 2021Fang Zhang, Weijun Jiang, Yating Liu, Liu Li, Rui Wang, Yang Libovine serum albumin at room temperature for 1 h, incubated with 216 anti-HCN4 primary antibodies (ab32675, Abcam, Cambridge, MA) at 4oC overnight, 217 incubated with HRP-labeled IgG secondary antibodies (ab6721,

(9) Conjugated activation of myocardial-specific transcription of Gja5 by a pair of Nkx2-5-Shox2 co-responsive elementsDevelopmental BiologySeptember 1, 2020Tianfang Yang, Zhen Huang, Hua Li, Linyan Wang, YiPing Chendescribed previously (Li et al., 2019). The primary antibodies used in this study were: anti-Hcn4 (ab32675, Abcam; 1:200), anti-Nkx2–5 (AF2444, Novus Biologicals; 1:200), anti-Cx40 (Cx40-A; Alpha Diagnostic

(10) ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node ConductionCirculation researchJuly 3, 2020Dai W, Nadadur RD, Brennan JA, Smith HL, Shen KM, Gadek M, Laforest B, Wang M, Gemel J, Li Y, et al.Catalog # 37–8900), β-catenin (Cell Signaling Technology, Catalog #9562), and HCN4 (Abcam, Catalog #ab32675) were used at 1:300 dilution. Mouse, Rat, and Rabbit HRP conjugated secondary antibodies were used at

(11) Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL‑1 cellsExperimental and Therapeutic MedicineOctober 25, 2019Jingjing Chen, Shunen Xu, Wei Li, Lirong Wu, Long Wang, Yongkang Li, Wei Zhouantibodies overnight at 4°C against Nkx2.5 (1:1,000; cat. no., ab106923; Abcam), HCN4 (1:1,000; cat. no., ab32675; Abcam), Cx40 (1:1,000; cat. no., ab16585; Abcam), SERCA2a (1:500; cat. no., ab2861; Abcam), ryanodine

(12) Adipose‑derived stem cells overexpressing SK4 calcium‑activated potassium channel generate biological pacemakersInternational Journal of Molecular MedicineOctober 16, 2019Mei Yang, Qingyan Zhao, Hongyi Zhao, Ankang Yang, Fengyuan Wang, Xi Wang, Yanhong Tang, Congxin HuangThe membranes were incubated with primary antibodies against HCN4 (rat monoclonal antibody, 1:1,000, ab32675, Abcam), SK4 (rabbit anti-rat monoclonal antibody, 1:500, ab215990, Abcam), phosphorylated extracellular

(13) Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell ResolutionCirculation researchAugust 2, 2019Goodyer WR, Beyersdorf BM, Paik DT, Tian L, Li G, Buikema JW, Chirikian O, Choi S, Venkatraman S, Adams EL, et al.(Alpha Diagnostics, Cx40-A) at 1:100 dilution; Anti-mouse Hcn4 Rat Monoclonal [SHG 1E5] antibody (Abcam, ab32675) at 1:75 dilution. The following secondaries were used at a 1:500 dilution: Donkey anti-goat IgG Alexa

(14) Nkx2-5 defines a subpopulation of pacemaker cells and is essential for the physiological function of the sinoatrial node in miceDevelopmentMarch 18, 2019Hua Li et al.described previously (Ye et al., 2015b). The primary antibodies used in this study were: anti-Hcn4 (ab32675, Abcam; 1:500), anti-Nkx2-5 (sc-8697, Santa Cruz; 1:500), anti-Tbx3 (sc-17871, Santa Cruz; 1:500), anti-Cx40

(15) A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cellsMolecular Medicine ReportsMarch 6, 2019Jian Zhang, Congxin Huangpotassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4; 1:1,000; cat. no. ab32675; Abcam, Cambridge, MA, USA), connexin 43 (Cx43; 1:3,000; cat. no. ab11370; Abcam) and connexin 45 (Cx45;

(16) Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cellsInternational Journal of Molecular MedicineNovember 27, 2018Jian Zhang, Mei Yang, An‑Kang Yang, Xi Wang, Yan‑Hong Tang, Qing‑Yan Zhao, Teng Wang, Yu‑Ting Chen, Cong‑Xin Huangantibodies against hyperpolarization-activated cyclic nucleotide-gated cation channel (HCN)4 (cat. no. ab32675), connexin (Cx)43 (cat. no. ab11370; both Abcam, Cambridge, MA, USA) and Cx45 (cat. no. F5108; Affinity

(17) Comparison of mouse brown and white adipose‑derived stem cell differentiation into pacemaker‑like cells induced by TBX18 transductionMolecular medicine reportsMay 1, 2018Sun AJ, Qiao L, Huang C, Zhang X, Li YQ, Yang XQ.no. ab9465) and hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4; rat; cat. no. ab32675) primary antibodies were purchased from Abcam (Cambridge, MA, UK). β-actin primary antibody (WB0196;

(18) Genetic targeting of Purkinje fibres by Sema3a-CreERT2Scientific reportsFebruary 5, 2018Li Y, Tian X, Zhao H, He L, Zhang S, Huang X, Zhang H, Miquerol L, Zhou B.ab6662, 1:200); TNNI3 (Abcam, ab56357, 1:100); Cx40 (Alpha Diagnostic, Cx40-A, 1:100); HCN4 (Abcam, ab32675, 1:100); tyrosine hydroxylase (TH, Millipore, AB152, 1:100); and Tuj1 (Covance, MMS-435P, 1:100). The

(19) Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cellsInternational Journal of Molecular MedicineNovember 16, 2017Yanjun Li, Mei Yang, Gege Zhang, Le Li, Bingjie Ye, Congxin Huang, Yanhong Tangrat anti-hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4) monoclonal antibody (ab32675, 1:500) and rabbit anti-connexin 43 (CX43) polyclonal antibody (ab11370, 1:2,000) (all Abcam, Cambridge,

(20) Involvement of Rictor/mTORC2 in cardiomyocyte differentiation of mouse embryonic stem cells in vitroInternational Journal of Biological SciencesJanuary 15, 2017Bei Zheng, Jiadan Wang, Leilei Tang, Chao Tan, Zhe Zhao, Yi Xiao, Renshan Ge, Danyan Zhu

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Anti-HCN4 antibody [SHG 1E5] from Abcam

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Anti-HCN4 antibody [SHG 1E5] from Abcam

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