Anti-Connexin 43 / GJA1 (phospho S368) antibody ab30559 from Abcam

Product Specs
ItemAnti-Connexin 43 / GJA1 (phospho S368) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab30559
Antibody TypePrimary
ClonalityPolyclonal
ApplicationsWestern blot
HostRabbit
Quantity100 UL
IsotypeIgG
Conjugate/TagUnconjugated
FormatPrepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns
ModificationsPhospho-S368
Gene Namegja1
ReactivityRat
TargetConnexin 43 / GJA1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) O-GlcNAcylation Inhibition Upregulates Connexin43 Expression in the Endothelium to Protect the Tight Junction Barrier in Diabetic RetinopathyInvestigative Opthalmology & Visual ScienceNovember 20, 2023Guodong Liu, Le Feng, Xiaoqiang Liu, Peng Gao, Fang Wangpurchased from Cell Signaling Technology (Danvers, MA, USA); primary Cx43 (ab11370/ab235585), phospho-Cx43 (ab30559), occludin (ab167161), zonula occluden-1 (ZO-1; ab221547), and GAPDH (ab8245) antibodies were purchased

(2) Glycated serum albumin decreases connexin 43 phosphorylation in the corpus cavernosumTranslational Andrology and UrologyNovember 1, 2022Yanping Huang, Wei Liu, Yidong Liu, Ming Zhang, Xiangguo Lv, Kai Hu, Shiran Xu, Mujun Luand incubated with antibodies against Cx43 (1:1,000; ab11370; Abcam, Cambridge, UK), p-Cx43 (1:1,000; ab30559; Abcam), eNOS (1:1,000; #32027; Cell Signaling Technology, Inc.), p-eNOS (1:1,000; sc-376542; Cell Signaling

(3) NF-κB and AP-1 are required for the lipopolysaccharide-induced expression of MCP-1, CXCL1, and Cx43 in cultured rat dorsal spinal cord astrocytesFrontiers in molecular neuroscienceJanuary 1, 2022Lu Y, Li B, Xu A, Liang X, Xu T, Jin H, Xie Y, Wang R, Liu X, Gao X, et al.,Santa Cruz,, Anti-Cx43,26980-1-AP,RRID:AB_2880711,WB/IF (1:1000/1:350),Rabbit,Proteintech Group,, Anti-p-Cx43 (Ser368),ab30559,RRID:AB_731707,WB (1:1000),Rabbit,Abcam,, Anti-β-actin,20536-1-AP,RRID:AB_10700003,WB (1:2000),Rabbit,Proteintech Group

(4) The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injuryNeural Regeneration ResearchJanuary 1, 2022Chun-Lei Shan, Zheng-Hui Zhao, Zheng-Hui Zhang, Ying-Jie Zhang, Wen-Jing Cheng, Pei-Jing Cheng, Pei-Jing Rongwith the following primary antibodies at 4°C overnight: phosphorylated-Cx-43 (rabbit; 1:1000; Cat# ab30559, Abcam (China), Shanghai, China) and Cx43 (rabbit; 1:500; Cat# ab87645, Abcam (China)). The membranes

(5) Propofol Mediated Protection of the Brain From Ischemia/Reperfusion Injury Through the Regulation of Microglial Connexin 43Frontiers in Cell and Developmental BiologyJune 8, 2021Tingting Zhang, Yanyan Wang, Qin Xia, Zhiyi Tu, Jiajun Sun, Qi Jing, Pei Chen, Xuan Zhaoin this study were Anti-CACNA1H (Cav3.2, ab128251, and Abcam), Anti-Connexin 43/GJA1 (phospho S368, ab30559, Abcam), Anti-Connexin 43/GJA1 antibody (ab230537, Abcam), Anti-Iba1 antibody (ab178846, Abcam), Gap27

(6) Novel Tocolytic Strategy: Modulating Cx43 Activity by S-NitrosationJournal of Pharmacology and Experimental TherapeuticsMarch 1, 2021Scott D. Barnett, Hazik Asif, Mitchell Anderson, Iain L. O. BuxtonLicor Biotechnology, Lincoln, NE). For pS368:S368 blots we used rabbit anti-pS368-Cx43 1° (1:1000, ab30559; Abcam) and IRDye 800-goat anti-rabbit 2° (1:25k, ab216773) followed by mouse anti-Cx43 polyclonal 1°

(7) Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxoloneJournal of neuroinflammationMarch 27, 2018Yin X, Feng L, Ma D, Yin P, Wang X, Hou S, Hao Y, Zhang J, Xin M, Feng J.Cx43(1:1000, Cell signaling technology, Cat#3512); Rabbit anti-phospho-Cx43/ GJA1(Ser 368)(1:1000, Abcam,Cat#ab30559); Rabbit anti-phospho-Cx43 (Ser373) (1:1000, Thermo Fisher Scientific, Cat#PA5-64670); Rabbit anti-phospho-Cx43(Ser265)(1:500,

(8) Breakthrough Cancer Pain Is Associated with Spinal Gap Junction Activation via Regulation of Connexin 43 in a Mouse ModelFrontiers in Cellular NeuroscienceJuly 17, 2017Xin Li, Siqing Jiang, Hui Yang, Qian Liao, Shousong Cao, Xuebin Yan, Dong Huangincubated with Cx43 mouse antibody (1:1000, Abcam, ab79010), p-Cx43 rabbit antibody (1:1000, Abcam, ab30559), GFAP mouse antibody (1:1000, Abcam, ab10062), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

(9) Gefitinib enhances oxaliplatin-induced apoptosis mediated by Src and PKC-modulated gap junction functionOncology reportsDecember 1, 2016Wu JF, Ji J, Dong SY, Li BB, Yu ML, Wu DD, Tao L, Tong XH.different from the oxaliplatin (OHP) group. (1:200) (ab25758), Cx43 (1:8,000) (ab11370), p-Cx43 (1:1,000) (ab30559) (Abcam, Cambridge, MA, USA), and mouse antibody against GAPDH (1:5,000) (60004-1-lg) (ProteinTech)

(10) Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion InjuryInternational Journal of Molecular SciencesJuly 22, 2016Shuai Hou, Ming-Ming Zhao, Ping-Ping Shen, Xiu-Ping Liu, Yuan Sun, Jia-Chun Fengantibodies to Cx43 (Abcam, Cambridge, MA, USA, ab11369, 1:2000), p-Cx43 (Abcam, Cambridge, MA, USA, ab30559, 1:2000), AKT (Abcam, Cambridge, MA, USA, ab25893, 1:500), p-AKT (Abcam, Cambridge, MA, USA, ab81283,

(11) Mechanism of Mitochondrial Connexin43′s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion InjuryInternational Journal of Molecular SciencesMay 5, 2016Shuai Hou, Ping-Ping Shen, Ming-Ming Zhao, Xiu-Ping Liu, Hong-Yan Xie, Fang Deng, Jia-Chun FengPKC antagonist (Ro-31-8425) were obtained from Millpore (St. Louis, MO, USA); Cx43 (ab11369), p-Cx43 (ab30559), β-actin (ab8227) , GAPDH (ab8245) and VDAC-1 (ab34726) antibodies were obtained from Abcam (Cambridge,

(12) High-dose insulin inhibits gap junction intercellular communication in vascular smooth muscle cellsMolecular Medicine ReportsJuly 1, 2015OU BIAN, HAISHAN ZHANG, QIGANG GUAN, YINGXIAN SUN, DINGYIN ZENGcells. bodies: Rabbit polyclonal anti‑Cx43 (cat. no. ab11370) or rabbit polyclonal anti‑p‑Cx43 (cat. no. ab30559; Abcam, Cambridge, MA, USA) at dilutions of 1:1,000 and 1:800, respectively. After washing with PBS,

(13) Aconitine mediates connexin43 and PKC phosphorylation status in cultured ventricular myocytes of neonatal ratsAfrican Journal of Pharmacy and PharmacologyMarch 31, 2011Yan LiuOR, USA) blocking for 30 min, cells were sequentially incubated with rabbit anti-Ser657 PKC (Abcam, ab30559; diluted 1:200) and rabbit anti-Ser657 phosphorylated PKC (Zymed, 13-8300; diluted 1:500) for 1 h. Fluorescein-conjugated

(14) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasionJournal of proteome researchNovember 5, 2010Yi-Ting Wang, Chia-Feng Tsai, Tzu-Chan Hong, Chih-Chiang Tsou, Pei-Yi Lin, Szu-Hua Pan, Tse-Ming Hong, Pan-Chyr Yang, Ting-Yi Sung, Wen-Lian Hsu, Yu-Ju Chen

(15) Aconitine alters connexin43 phosphorylation status and [Ca2+] oscillation patterns in cultured ventricular myocytes of neonatal ratsToxicology in vitro : an international journal published in association with BIBRADecember 1, 2007Zhang SW, Liu Y, Huang GZ, Liu L.

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Anti-Connexin 43 / GJA1 (phospho S368) antibody from Abcam

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Anti-Connexin 43 / GJA1 (phospho S368) antibody from Abcam

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