HID1 Antibody (OTI2F4) NBP2-02667 from Novus Biologicals, a Bio-Techne Brand

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Scientific reports
December 02 2022

Fig 1: Levels and localization of DCV proteins are affected in Vti1a/b DKO neurons. (a) Representative examples of neurons immunostained for CgB. (b–d) CgB normalized staining intensity is increased in the Golgi (Mann Whitney test: **p = 0.0024) (b), not significantly different in the soma (t-test: ns, p = 0.1142) (c), and increased in the soma excluding Golgi (t-test: **p = 0.0056) (d) (DHZ: n = 22; DKO: n = 19). (e) Representative examples of neurons immunostained for HID1. (f–h) HID1 normalized staining intensity is decreased in the Golgi (t-test: ****p < 0.0001) (f), soma (Mann Whitney test: ****p < 0.0001) (g), and soma excluding Golgi (Mann Whitney test: ***p = 0.0001) (h) (DHZ: n = 32; DKO: n = 35). (i) Representative examples of neurons immunostained for CCDC186. (j–l) CCDC186 normalized staining intensity is not significantly different in the Golgi (t-test: ns, p = 0.2927) (j), but decreased in the soma (t-test: ****p < 0.0001) (k), and soma excluding Golgi (t-test: ***p = 0.0006) (l) (DHZ: n = 28; DKO: n = 27). (m) Representative examples of neurons expressing NPY-SEP. (n–p) NPY-SEP normalized fluorescence intensity is not significantly different in the Golgi (Mann Whitney test: ns, p = 0.0567) (n), but decreased in the soma (Mann Whitney test: **p = 0.0016) (o), and soma excluding Golgi (Mann Whitney test: *p = 0.0111) (p) (DHZ: n = 67; DKO: n = 64). Bars show mean ± SEM. Detailed statistics are shown in Supplementary Table 1. Scale bar is 2 μm.
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Scientific reports
February 18 2023

Fig 2: Several Golgi proteins overlap with TGN38. (a–c) Representative examples of neurons immunostained for a cis-/medial Golgi marker (GM130 or giantin), TGN38 and a protein of interest (ARL1, SMIM7, HID1 and TMEM87A). Images showing immunostaining of cis-/medial Golgi marker and TGN (a), TGN and protein of interest (b), cis-/medial Golgi and protein of interest (c). (d) Zoom from the white square area marked in (c), showing all three stained proteins. White line is a representative example of a line drawn for intensity plotting. (e) Distance between intensity peaks of TGN38 and protein of interest, cis-/medial Golgi and TGN; and cis-/medial Golgi and protein of interest, measured by line scans. ARL1: (TGN38-ARL1: n = 15; Giantin-TGN38: n = 15; Giantin-ARL1: n = 15). One-way ANOVA with Tukey’s multiple comparisons test: TGN38-ARL1 vs Giantin-TGN38 and TGN38-ARL1 vs Giantin-ARL1: ****p < 0.0001, Giantin-TGN38 vs Giantin-ARL1: non-significant (ns). SMIM7: (TGN38-SMIM7: n = 15; GM130-TGN38: n = 15; GM130-SMIM7: n = 15). One-way ANOVA with Tukey’s multiple comparisons test: TGN38-SMIM7 vs GM130-TGN38 and TGN38-SMIM7 vs GM130-SMIM7: ****p < 0.0001, GM130-TGN38 vs GM130-SMIM7: non-significant (ns). HID1: (TGN38-HID1: n = 16; Giantin-TGN38: n = 16; Giantin-HID1: n = 16). One-way ANOVA with Tukey’s multiple comparisons test: TGN38-HID1 vs Giantin-TGN38 and TGN38-HID1 vs Giantin-HID1: ****p < 0.0001, Giantin-TGN38 vs Giantin-HID1: non-significant (ns). TMEM87A: (TGN38-TMEM87A: n = 15; GM130-TGN38: n = 15; GM130-TMEM87A: n = 15). One-way ANOVA with Tukey’s multiple comparisons test: TGN38-TMEM87A vs GM130-TGN38 and TGN38-TMEM87A vs GM130-TMEM87A: ****p < 0.0001, GM130-TGN38 vs GM130-TMEM87A: non-significant (ns). Dotted lines represent average distance between TGN38 and GM130 or TGN38 and giantin in the entire dataset. Bars show mean ± SEM. Detailed statistics are shown in Supplementary Table 1. Scale bar is 3 µm (a) and 1 µm (d).