Fig 1: BHD suppressed RANKL-stimulated osteoclastogenesis-related protein and gene expression (A–D) The protein levels of NFATc1, c-fos and Ctsk of Raw 264.7cells pretreated with or without BHD for 0,1,3,5 days. BHD suppressed RANKL-stimulated protein expression levels increase in a time-dependent manner. (E–H) The mRNA levels of NFATc1, Ctsk, V-ATPase-d2 and Acp5 of Raw 264.7cells pretreated with or without BHD. BHD suppressed RANKL-stimulated mRNA expression levels increase of these genes. Columns in the charts were presented in a manner of mean ± SD. (n = 3, three independent experiments). *p<0.05, **p<0.01, ***p<0.001.