Fig 1: AK021443 overexpression induces extracellular matrix deposition. Protein expression levels were (A) determined by western blotting and (B) semi-quantified for TIMP1, collagen1, ET-1, MMP2 and MMP9. ***P<0.001 vs. pcDNA. TIMP1, TIMP metallopeptidase inhibitor 1; ET-1, endothelin 1; MMP, matrix metallopeptidase.
Fig 2: MiR-1 inhibits the expression of VEGF-A and EDN1 3′ untranlated region (UTR)-integrated luciferase reporter genes. a The target sites for miR-1 in the VEGF-A (NM_001025369.2), and EDN1 (NM_001955.4) 3′ UTR were identified with the TargetScan database. b Luciferase reporter assay using the vector encoding the full-length 3′ UTR of VEGF-A, and EDN1 3′ UTR messenger RNA. The Renilla luciferase values were used to normalize firefly luciferase values. Cont. control, Mut mutant, Wt wild type, *P < 0.05, **P < 0.01
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