Fig 1: Effect of CEACAM6 knockdown on wound healing. (A) Wound-healing analysis of cell migration after transfection of CEACAM6 siRNA and NC siRNA in GBC-SD and SGC-996 cells (magnification, ×50). (B) Relative migration is shown in relevant graph bars. **P<0.01 vs. NC group. CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; NC, negative control; siRNA, small interfering RNA.
Fig 2: Effect of depletion of CEACAM6. (A) Changes over 3 days in GBC-SD and SGC-996 cells measured by CCK8 assay. (B and C) Colony formation analysis of the cell colony number after transfection with CEACAM6 siRNA and NC siRNA in GBC-SD cells and in SGC-996 cells. *P<0.05, **P<0.01 vs. NC group. CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; NC, negative control; siRNA, small interfering RNA.
Fig 3: CEACAM6 staining (A) Negative expression of CEACAM6 in peritumoral tissue. (B) Positive expression of CEACAM6 in well-differentiated gallbladder carcinoma tissue. (C) Positive expression of CEACAM6 in moderately differentiated gallbladder carcinoma tissue. (D) Positive expression of CEACAM6 in poorly differentiated gallbladder carcinoma tissue. Magnification, ×200. CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6.
Fig 4: Normal tissue-derived organoids exhibited some tumor-like features at the transcriptome level. A Expression patterns of differentially expressed genes (DEGs) of in vivo tumor and adjacent normal tissues. Colors from blue to red represent low to high expression. Short, short-term culture; Long, long-term culture. Patient number in black, blue, and orange represent cells collected from in vivo tissues, in vitro chemical-defined medium, and conditioned medium. The detail description for some of these genes can be found in Additional file 4: Table S3. Many of tumor highly specifically expressed genes were reported to play important roles in colorectal cancer metastasis and progression (such as TGFBI, SCD, TESC, CEACAM6), while some of normal tissue highly expressed genes were well-known intestinal cell-type-specific marker genes, such as SI, CA1, and PYY. B Immunostaining of CEACAM6 in the adjacent normal and tumor tissues in vivo. Scale bar, 100 µm. C Immunostaining of CEACAM6 in the in vitro normal-tissue- and tumor-derived organoids. Scale bar, 50 µm
Fig 5: Expression levels of the three key genes in different HNSCC tissues. A Real-time PCR of CEACAM5, CEACAM6 and CLCA4 in laryngeal carcinoma, hypopharyngeal carcinoma and oropharyngeal carcinoma. The relative mRNA expression levels of these three genes in most HNSCC tissues were lower than those in adjacent noncancerous tissues (scale bars represent the mean ± SEM, Student’s t-test, *P < 0.05, **P < 0.01). B IHC of CEACAM5, CEACAM6 and CLCA4 in laryngeal carcinoma, hypopharyngeal carcinoma and oropharyngeal carcinoma. The protein expression levels of these three genes were lower than those in adjacent noncancerous tissues. C Semiquantification analysis of immunohistochemistry of each laryngeal carcinoma, hypopharyngeal carcinoma and oropharyngeal carcinoma tissue and paired adjacent normal tissue. Most of the tumour tissues in this study showed less staining than adjacent normal tissues. Abbreviations: LC: laryngeal carcinoma; HPC: hypopharyngeal carcinoma; OPC: oropharyngeal carcinoma
Supplier Page from Abcam for Anti-CEACAM6 antibody [EPR4403]