Fig 1: IL-32 protein half-life is regulated by the oxygen sensor ADO. (A) JJN-3 cells were transfected with ADO- and nontargeting Ctrl siRNA. After 24 h, the cells were seeded and cultured overnight in normoxia or hypoxia before being treated with 5 µg/ml CHX and the IL-32 CHX chase assay in normoxia and hypoxia. One representative WB of IL-32 and ADO siRNA-treated cells of n = 5 independent experiments is shown. (B) JJN-3 cells were transfected with ADO and nontargeting Ctrl siRNA. After being transfected for 24 h, the cells were cultured overnight in hypoxia before being treated with 5 µg/ml CHX and reoxygenized in normoxic culture conditions. Cells were harvested at indicated time points.
Fig 2: Time course of induction of known hypoxia-inducible proteins. Five cell lines; SH-SY5Y (A), RKO (B), HepG2 (C), Kelly (D), and EA.hy926 (E) were exposed to 1% O2 for the indicated periods of time. Representative immunoblots (top) and mRNA levels of the ADO substrates (RGS5, RGS4, and IL-32) are shown below. All data represent the mean ± SD from three independent experiments.
Supplier Page from Abcam for Anti-ADO antibody [EPR6581]