Fig 1: Molecular analysis of the effects of JOA on differentiation-related genes in Kasumi-1 and NB4 cells. (A) Effect of JOA on the mRNA expression of CD64, HLA-DPA1, HLA-DRA, and CDKN2A in Kasumi-1 cells and c-MYC, G-CSFR, and CCND1 in NB4 cells. The cells were incubated with 1 µM of JOA for 24, 48, or 72 h. (B) Effect of JOA on protein expression of CD64, HLA-DP, and CDKN2A in Kasumi-1 cells, and c-MYC, G-CSFR, and CCND1 in NB4 cells. The cells were incubated with JOA (0.25, 0.5, or 1 µM) or Ara-C (1 µM) for 72 h. (C) Graph bars showing the protein expression quantified using an AI600 imager (*p < 0.05 and **p < 0.01).
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