Fig 1: miRNA-673-5p antagomir stimulates myelin gene expression in newborn rat sciatic nerves. One-day old newborn rats were received miR-673-5p antagomir via hypodermic injection at the dosage of 5 nmol/rat every 2 days. After treatment, the sciatic nerves were collected at different time points as indicated and subjected to further analysis. A, Tsc2, Oct6, Krox20, Mbp, Mpz, and Pmp22 expressions were analyzed by qRT-PCR. 18S rRNA was used as an internal control. B, the cross-sections of sciatic nerves were immunostained for Krox20 and MPZ. Scale bar = 50 μm. C, the fluorescent intensity for KROX20 and MPZ as shown in (B). Data are expressed as means ± SD. Statistical significance was analyzed by Student t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. qRT-PCR, quantitative real-time PCR.
Supplier Page from Abcam for Anti-Myelin Protein Zero antibody