Fig 1: Effects of PTPI22 on p130Cas phosphorylation and interactions among p130Cas, Crk, paxillin and DOCK180.(A) The tyrosine phosphorylation level of p130Cas was detected by immunoprecipitation with P-Tyr-100 followed by western blotting. (B) Interactions among p130Cas, Crk and paxillin were detected by immunoprecipitation of Crk followed by western blotting detections. (C) Confocal microscopy images showing the effect of PTPI22 on intracellular translocation of Crk toward the cell periphery and the plasma membrane (arrows). (D) Interaction between Crk and DOCK180 was detected with double immunofluorescence labelling. Treatment with PTPI22 increased co-localisation of Crk and DOCK180 (arrows). (E) Effect of the Src inhibitor PP2 (200 nM) on PTPI22-stimulated EC migration. Data were mean ± S.E.M.; *P < 0.05 vs control, unpaired t-test or one-way ANOVA, n = 3–4.