Fig 1: DTNBP1 genotype differences in pY1472-GluN2B phosphorylation, Fyn, and PLC? in PSD enrichments of the frontal cortex and hippocampus. a, e, i, m Main effects of DTNBP1 genotype on the pY1472-GluN2B:total GluN2B ratio, abundance of Fyn, pY527-Src phosphorylation, and abundance of PLC? in PSD enrichments of the frontal cortex. b, f, j, n Developmental trajectories of pY1472-GluN2B:total GluN2B ratio, abundance of Fyn, pY527-Src phosphorylation, and abundance of PLC? in mice of different DTNBP1 genotypes in the PSD of the frontal cortex. c, g, k, o Main effects of DTNBP1 genotype on the pY1472-GluN2B:total GluN2B ratio, abundance of Fyn, pY527-Src phosphorylation, and abundance of PLC? in PSD enrichments of the hippocampus. d, h, l, p Developmental trajectories of pY1472-GluN2B:total GluN2B ratio, abundance of Fyn, pY527-Src phosphorylation, and abundance of PLC? in mice of different DTNBP1 genotypes in the PSD of the hippocampus. *p < 0.05; ***p = 0.0005. N.S. not significant. Error bars represent SEM
Fig 2: Effects of DTNBP1 genotype on the GluN2B-GluN2A switch in the frontal cortex and hippocampus. a, e, i Main effects of DTNBP1 genotype [wild-type (+/+), heterozygous DTNBP1 mutant (+/-), and homozygous DTNBP1 null mutant (-/-)] on GluN2B:GluN2A, GluN2B:GluN1, and GluN2A:GluN1 ratios in PSD enrichments of the frontal cortex. b, f, j Developmental trajectories of GluN2B:GluN2A, GluN2B:GluN1, and GluN2A:GluN1 ratios in wild-type (+/+), heterozygous DTNBP1 mutant (+/-), and homozygous DTNBP1 null mutant (-/-) mice in the PSD of the frontal cortex. c, g, k Main effects of DTNBP1 genotype [wild-type (+/+) and homozygous DTNBP1 null mutant (-/-)] on GluN2B:GluN2A, GluN2B:GluN1, and GluN2A:GluN1 ratios in PSD enrichments of the hippocampus. d, h, l Developmental trajectories of GluN2B:GluN2A, GluN2B:GluN1, and GluN2A:GluN1 ratios in wild-type (+/+) and homozygous DTNBP1 null mutant (-/-) mice in the PSD of the hippocampus. Images of representative blots for all hippocampal proteins are provided in Additional file 1: Figure S3. For bar graphs in panels a, c, e, g, i, and k, data are collapsed across age groups. Refer to Table 2 for sample sizes. PSD postsynaptic density, cSM crude synaptic membrane, FCx frontal cortex, N.S. not significant. *p < 0.05. Error bars represent SEM
Fig 3: Absence of DTNBP1 genotype differences in scaffolding proteins in PSD enrichments of the frontal cortex and hippocampus. a, e Main effects of DTNBP1 genotype on abundance of PSD-95 and SAP102 in PSD enrichments of the frontal cortex. b Developmental trajectories of PSD-95 in wild-type (+/+), heterozygous DTNBP1 mutant (+/-), and homozygous DTNBP1 null mutant (-/-) mice in the PSD of the frontal cortex. f Developmental trajectories of SAP102 in wild-type (+/+) and homozygous DTNBP1 null mutant (-/-) mice in the PSD of the frontal cortex. c, g Main effects of DTNBP1 genotype [wild-type (+/+) and homozygous DTNBP1 null mutant (-/-)] on abundance of PSD-95 and SAP102 in PSD enrichments of the hippocampus. d, h Developmental trajectories of PSD-95 and SAP102 in wild-type (+/+) and homozygous DTNBP1 null mutant (-/-) mice in the PSD of the hippocampus. PSD postsynaptic density, cSM crude synaptic membrane, FCx frontal cortex, N.S. not significant. Error bars represent SEM
Fig 4: Representative images of Western blots quantified by Odyssey infrared imaging in this study (a–j). M male, F female, kDa kilodaltons, m marker, -/- DTNBP1 null, +/- DTNBP heterozygous, +/+ wild-type
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