Fig 2: PTE pre-treatment regulates the NF-?B pathway in LPS induced ALI through NR4A1. The expression of NR4A1 in lung tissues was examined by (A) immunohistochemistry (scale = 100 µm) and (B) western blotting. (C) The expression of p-p65, p65, p-I?Ba, and I?Ba was analyzed by western blotting. GAPDH was used as an internal control. *p < 0.05, **p < 0.01 vs. control group. #p < 0.05 vs. ALI group. H-PTE: high dose of PTE (40 mg/kg); HY-N2148: NR4A1 agonist (Cytosporone B, 50 µL, 1 µM); NF-?B: nuclear factor-?B; ALI: acute lung injury.

Fig 3: The binding activity of PTE to NR4A1, and survival rate in this study. (A) The binding activity of PTE to NR4A1 was forecasted using UCSF chimaera and AutoDock Vina softwares. There is a good binding activity of PTE to NR4A1 (total score = -6.7). (B) The survival rate was tracked every 6 h after the LPS instillation. The percent survival was expressed as a Kaplan-Meier survival curve.

Fig 4: Effect of PTE pre-treatment on NR4A1 expression in LPS induced ALI rats. The expression of NR4A1 was analyzed by (A) immunohistochemistry (scale = 100 µm) and (B) western blotting in the lung tissues of each group. *p < 0.05, **p < 0.01 vs. control group. #p < 0.05, ##p < 0.01 vs. ALI group. &p < 0.05 vs. L-PTE + LPS group. L-PTE, low dose of PTE (10 mg/kg); M-PTE, medium dose of PTE (20 mg/kg); H-PTE, high dose of PTE (40 mg/kg); ALI, acute lung injury.

Fig 5: Effect of sevoflurane anesthesia during pregnancy on proteins related to the NR4A1/NF-?B pathway in the hippocampi of offspring rats. A Western blot analysis of NR4A1, p-P65, P65, p-I?Ba, and I?Ba protein; B Relative expression of NR4A1; C Relative expression of p-P65/P66; D Relative expression of p-I?Ba/I?Ba. There were 6 rats in each group, and the data are represented as \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\overline{X}$$\end{document}X¯ ± SD. *p < 0.05 compared to the control group