Fig 2: Changed expression of the Na,K-ATPase a isoforms in the heart from a2 +/G301R mice but similar expression of the Na,Ca-exchanger-1.The hearts from 3- and 8-month-old a2 +/G301R mice showed reduced expression of the Na,K-ATPase a2 isoform (A, n=7; D, n=6), increased expression of Na,K-ATPase a1 isoform (B, n=7; E, n=5 to 6), and no change in the expression of Na,Ca-exchager-1 (NCX1; C, n=7; F, n=12) in comparison with wild type (WT). The expression profile was the same in hearts of 3-month-old (A–C) and 8-month-old (D–F) mice. Upper part of each panel shows representative Western blot bands for the averaged data shown below. The representative bands are shown from the same membrane, as well as the molecular marker shown to the right. The images cropped to include molecular weights from 250 to 50 kDa, as indicated. Total protein load was detected with the stain-free gel, and the corresponding representative bands were cropped to the same molecular weight range. Protein expression was compared using unpaired t-test. *P<0.05 and **P<0.01.