Fig 1: Quantification of MUC5AC-positive spots in bronchial cultures from different donors at weeks 5, 7, and 9 after air-lift. (A) The number of mucin 5 AC-positive spots visible in tissues stained with a MUC5AC primary antibody was measured by using the CellInsight™ CX7 high-content screening platform. Data are presented as mean ± SEM for 3 (donors BR1 and BR2) or 2 technical replicates (donors BR3–BR5) per time point. (B) Variance components were estimated in bronchial cultures. The relative contributions of the donor and time (week), as well as those of residual factors, were estimated in the variance analysis. MUC5AC = mucin 5 AC; SEM = standard error of the mean.