The Insulin Antibody from ImmunoStar, Inc. is a Rabbit Polyclonal antibody to Dynll1, INS, INS-IGF2, Ins1, Insulin, insulin I, and LC8. This antibody recognizes Human, Mouse, Rat, Bovine, Chicken/Bird, Drosophila/Arthropod, Horse, Non-Human Primate, Porcine, Reptile, and Sheep antigen. The Insulin Antibody has been validated for the following applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot.
Description
The Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/3000-1/5000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum