Fig 1: CD70+ Lymphoma Cells Induce Downregulation of CD27 or CD28 Expression on Intratumoral T Cells, which Impairs Their Capacity to Proliferate(A) Histograms showing CD27 or CD28 expression of CD27+CD28+ T cells cultured in anti-CD3-coated plate plus anti-CD28 Ab for 2 or 5 days (activated). Cells cultured in uncoated plate were used as control (resting).(B) CD27 or CD28 expression from CD4+ T cells cultured in anti-CD3-coated plate plus anti-CD28 Ab in the presence or absence of TGF-β for 3 days.(C) Immunohistochemistry showing CD70 expression from FL (n = 8) and tonsil tissue.(D) Plots showing CD70 expression on CD19+ cells from representative lymphoma (n = 11) and tonsil tissues.(E) Histograms showing (i) CD70 expression on DoHH2 cells, (ii) expression of CD27 and CD28 on T cells co-cultured with DoHH2 cells for 3 days, (iii) CD70 expression of DoHH2 cells treated with either anti-CD70 blocking Ab or mIgG for 2 h, and (iv) expression of CD27 on CD4+ T cells co-cultured with DoHH2 cells pretreated with either anti-CD70 blocking Ab or mIgG for 3 days.(F) Histograms showing CFSE staining of CD27+CD28+, CD27+CD28−, CD27−CD28+, or CD27−CD28− T cells cultured in anti-CD3-coated plate plus anti-CD28 Ab for 3 days. Graph shows the numbers of CFSEdim cells of these subsets; n = 4.(G) Plots showing CD45RO and CCR7 expression from CD4+CD27− or CD4+CD27+ T cells. Cells in box represent terminally differentiated T cells (CD45RO−CCR7−; TEMRA). See also Figure S7.