Cya A (3D1) Antibody sc-13582 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemCya A (3D1) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-13582
Antibody TypePrimary Antibodies
ApplicationsWB
ReactivityB. pertussis
Clone3D1
Quantity200 µg/ml
ClonalityMonoclonal
Conjugate/TagUnconjugated
Hostmouse
FormatPurified
TargetCya A
IsotypeIgG1

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Characterization of DNA Processing Protein A (DprA) of the Radiation-Resistant Bacterium Deinococcus radioduransMicrobiology SpectrumDecember 21, 2022Dhirendra Kumar Sharma, Hari S. Misra, Ishu Soni, Yogendra S. Rajpurohitworking protocols were used, as described previously (51). The antibody against the T18 (catalog number SC-13582) domain of the CyaA protein of Bordetella pertussis was procured from Santa Cruz Biotechnology, Inc.,

(2) Identification of a Family of Vibrio Type III Secretion System Effectors That Contain a Conserved Serine/Threonine Kinase DomainmSphereAugust 25, 2021N. Plaza, I. M. Urrutia, K. Garcia, M. K. Waldor, C. J. Blondelthe following dilutions: anti-CyaA (mouse monoclonal, 1:2,000; Santa Cruz Biotechnology catalog no. sc-13582), anti-RpoB (rabbit monoclonal, 1:2,000; Abcam catalog no. ab191598), anti-mouse IgG conjugated to horseradish

(3) A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® CloningMethods in Molecular BiologyJuly 14, 2021Macy G. Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, Scot P. OuelletteAntibody: Anti-CyaA T25 fragment. Antibody: Anti-CyaA T18 region (Santa Cruz biotechnology cat. # sc-13582). 2.4 BACTH Reporter Strains and E. coli Cloning Strains For the BACTH system, three E. coli adenylate

(4) PprA Protein Inhibits DNA Strand Exchange and ATP Hydrolysis of Deinococcus RecA and Regulates the Recombination in Gamma-Irradiated CellsFrontiers in Cell and Developmental BiologyApril 20, 2021Yogendra Singh Rajpurohit, Dhirendra Kumar Sharma, Hari S. Misramolecular biology techniques were used as described (Green and Sambrook, 2012). Antibodies against the T18 (SC-13582) and T25 (SC-33620) domains of CyaA of Bordetella pertussis were procured commercially (Santa Cruz Biotechnology, Inc

(5) Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complexNature CommunicationsDecember 1, 2020Hyunmin Kim, Tomoko Kubori, Kohei Yamazaki, Mi-Jeong Kwak, Suk-Youl Park, Hiroki Nagai, Joseph P. Vogel, Byung-Ha Oheach Legionella strain expressing a CyaA-tagged effector were used. Anti-CyaA mouse monoclonal IgG (sc-13582, Santa Cruz Biotechnology, Inc.) diluted to 1:3000 and goat anti-mouse IgG (#31430, Invitrogen) conjugated

(6) PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthusNature communicationsOctober 7, 2020Treuner-Lange A, Chang YW, Glatter T, Herfurth M, Lindow S, Chreifi G, Jensen GJ, Søgaard-Andersen L.were used to detect GFP-tagged proteins (Roche; 11814460001) and CyaA-tagged proteins (Santa Cruz, sc-13582). As secondary antibodies, goat anti-rabbit immunoglobulin G peroxidase conjugate (Sigma-Aldrich, A8275)

(7) YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coliNature CommunicationsDecember 17, 2019Elsa Germain, Paul Guiraud, Deborah Byrne, Badreddine Douzi, Meriem Djendli, Etienne Maisonneuvebuffer for 1 h (PBST + 5% milk). The first anti-cyaA antibody has been diluted 1/5.000 (Santa Cruz, ref: sc-13582) in PBST milk and incubated for 1 h at room temperaure. The secondary antibody (Sigma, ref: A4416) has

(8) Synthetic hydrophobic peptides derived from MgtR weaken Salmonella pathogenicity and work with a different mode of action than endogenously produced peptidesScientific ReportsDecember 1, 2019Mariana Rosas Olvera, Preeti Garai, Grégoire Mongin, Eric Vivès, Laila Gannoun-Zaki, Anne-Béatrice Blanc-Potardantibody (Tebubio) was used at 1:5000 dilution, whereas mouse anti-T18 antibody (monoclonal antibody sc-13582, Santa-Cruz) was used at 1:1000 dilution. Rabbit anti-OmpF and anti-OmpC antibodies were used at 1:1000.

(9) Interaction of ArmZ with the DNA-Binding Domain of MexZ Induces Expression of mexXY Multidrug Efflux Pump Genes and Antimicrobial Resistance in Pseudomonas aeruginosaAntimicrobial Agents and ChemotherapyDecember 1, 2019Adam Kawalek, Magdalena Modrzejewska, Bartlomiej Zieniuk, Aneta Agnieszka Bartosik, Grazyna Jagura-BurdzyWestern blot analysis of CyaT18-fused proteins was performed by the use of mouse anti-CyaA antibodies (3D1, catalog no. SC-13582; Santa Cruz Biotechnology). For Western blot analysis, cell pellets were resuspended in loading buffer

(10) Identification and characterization of a large family of superbinding bacterial SH2 domainsNature CommunicationsDecember 1, 2018Tomonori Kaneko et al.were detected by western blot, using mouse anti-CyaA antibody (Santa Cruz Biotechnology, catalog # sc-13582) diluted 1:5000 and goat anti-mouse IgG-HRP conjugate (Bio-Rad, catalog # 170-6516) diluted 1:3000. Protein expression

(11) Phosphorylation of FtsZ and FtsA by a DNA Damage-Responsive Ser/Thr Protein Kinase Affects Their Functional Interactions in Deinococcus radioduransmSphereAugust 29, 2018Ganesh K. Maurya, Kruti Modi, Manisha Banerjee, Reema Chaudhary, Yogendra S. Rajpurohit, Hari S. Misraused in this study are listed in Table S1 in the supplemental material. Antibodies against the T18 (SC-13582) and T25 (SC-33620) domains of CyaA of Bordetella pertussis were procured commercially (Santa Cruz Biotechnology, Inc

(12) Endogenous and Exogenous KdpF Peptide Increases Susceptibility of Mycobacterium bovis BCG to Nitrosative Stress and Reduces Intramacrophage ReplicationFrontiers in Cellular and Infection MicrobiologyApril 6, 2017Mariana Rosas Olvera, Eric Vivès, Virginie Molle, Anne-Béatrice Blanc-Potard, Laila Gannoun-Zakinitrocellulose membrane (Invitrogen) for immunoblotting. Mouse anti-T18 antibodies (monoclonal antibody sc-13582, Santa-Cruz) was used at 1:1000 dilution. Mouse anti-DnaK antibody (Tebubio) at 1:5000 dilution was

(13) ClpP-deletion impairs the virulence of Legionella pneumophila and the optimal translocation of effector proteinsBMC MicrobiologyDecember 1, 2016Bei-bei Zhao, Xiang-hui Li, Yong-lun Zeng, Yong-jun Lumutant (Xp02) probed with monoclonal antibody specific to the CyaA epitope (Santa Cruz Biotechnology sc-13582) and ICDH with anti-ICDH antibody (a kind gift with Dr. Vogel JP). (TIF 3783 kb) Abbreviations CFU

(14) Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatisFrontiers in Cellular and Infection MicrobiologyFebruary 11, 2015Emilie Gauliard, Scot P. Ouellette, Kelsey J. Rueden, Daniel LadantTTBS buffer, and finally incubated with a mouse monoclonal antibody 3D1 (Santa Cruz Biotechnology; sc-13582; it specifically recognizes the T18 fragment), diluted 1:1000 in TTBS buffer containing 1% milk powder

(15) Role ofBordetella pertussisRseA in the cell envelope stress response and adenylate cyclase toxin releasePathogens and DiseaseAugust 22, 2013Tomoko Hanawa, Hideo Yonezawa, Hayato Kawakami, Shigeru Kamiya, Sandra K. Armstrongdilution of mouse monoclonal antibody specific for mouse monoclonal antibody to the B. pertussis CyaA (sc-13582; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). After reaction with a goat anti-mouse IgG and

(16) Reassessing the Role of DotF in the Legionella pneumophila Type IV Secretion SystemPLoS ONEJune 7, 2013Molly C. Sutherland, Kelsey A. Binder, Phillip Y. Cualing, Joseph P. Vogelsamples were collected and protein levels were ascertained by western blot with a CyaA specific antibody (sc-13582, Santa Cruz Biotechnology). Quantitative Assay for Protein-protein Interaction (β-galactosidase Activity) E. coli

(17) The Campylobacter jejuni CiaC virulence protein is secreted from the flagellum and delivered to the cytosol of host cellsFrontiers in Cellular and Infection MicrobiologyMarch 20, 2012Jason M. Neal-McKinney, Michael E. Konkelwere transferred to PVDF membranes and probed with a 1:1000 dilution of a mouse anti-ACD antibody (sc-13582, Santa Cruz Biotechnology). Bound mouse antibody was detected using 1:2000 diluted rabbit anti-mouse

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Cya A (3D1) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Cya A (3D1) Antibody from Santa Cruz Biotechnology, Inc.

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