Fig 2: Visualization of MAPK3 expressed in ovarian granulosa cells of the PCOS, BSTJF and control groups by double-labeling immunofluorescence with FoxL2 (winged helix for head transcription factor gene2, specific expression in adult ovarian granulosa cells) (green) and MAPK3 (red). Nuclei were counterstained with 40,6-diamidino-2-phenylindole (DAPI) (blue fluorescence). All scale bars are 100 µm. The expression ratio of the target protein is shown by bar plots (n = 4 in each group). Data were presented as the mean ± SEM. One-way ANOVA for multiple comparisons and LSD test for pairwise comparisons between groups

Fig 3: (A) The expression levels of piR-121380 and PTPN7 in fresh, frozen–thawed, and capacitated sperm. **p < 0.01. (B) The expression levels of ERK1 and ERK2 in fresh, frozen–thawed, and capacitated sperm. **p < 0.01. *p < 0.05. The data are representative of at least three independent experiments (mean ± SEM).

Fig 4: Localization of ERK1/2 and PTPN7 on boar sperm. (A) ERK1, (B) ERK2, and (C) PTPN7.

Fig 5: Farrerol inhibits Ang II-induced cardiac remodeling in vitro. (A) Representative images of Myocardial cells stained with a-actinin in each group and the relative value of the statistical chart; (B) Mitochondrial ROS levels in NRVMs were determined by MitoSOX Red staining; (C) The expression of p-AKT (Ser473), AKT, NOX2 and ß-actin detected by Western Blot; (D) The expression of p-ERK 1/2, ERK 1/2, p-STAT3, STAT3, NFAT2 and ß-actin detected by Western Blot; (E) the migration ability of fibroblasts by Wound scratch assay in each group; (F) The proliferation ability of fibroblasts was detected by CCK-8; (G) The expression of p-AKT (Ser473), AKT, NOX2 and ß-actin detected by Western Blot (Salline = 3, Farrerol = 3, Ang II = 3, Ang II + Farrerol = 3).