Fig 1: Effects of overexpression of candidate miRNAs asssociated with amelogenesis imperfecta on ameloblast differentiation. (A) Schematic of the experiment. (B) Gene expression of the indicated genes after treatment with the mimic for the indicated miRNA in mHAT9d cells (n = 6). **p < 0.01; ***p < 0.001. (C) Immunoblotting for AMELX, KLK4, MMP20, and GAPDH (internal control) in mHAT9d cells under the indicated conditions. Graph shows the quantification of the immunoblotting. n = 3 per group. *p < 0.05; **p < 0.01; ***p < 0.001. (D) ICC for AMELX in mHAT9d cells under the indicated conditions. Scale bar, 50 µm. Graph shows the quantification of images from four independent experiments. **p < 0.01; ***p < 0.001.