Fig 1: Increased GDH and GPBB in Psmc1fl/fl;CaMKIIa-Cre forebrain. (A) Representative immunoblots and quantification of GDH and GPBB in mitochondria and cytosol respectively purified from control and Psmc1fl/fl;CaMKIIa-Cre cortices at 6 weeks-old. Pyruvate dehydrogenase E1 component subunit alpha (PDHE1a; mitochondria) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cytosol) were used as a loading controls. Images were cropped from those shown in Supplementary Figure 6. Mean ± SEM of n = 3 mice. *p < 0.05 and **p < 0.01 by unpaired Students t-test. (B) Immunostaining of cortical and hippocampal brain sections from control and Psmc1fl/fl;CaMKIIa-Cre mice at 6 weeks-old with GDH and GPBB. Asterisks and arrows indicate astrocytes and Lewy-like inclusions respectively. Scale bar = 50 µm.