Fig 1: Morphine-induced hnRNP K protein expression in mouse CNS. Morphine increased hnRNP K protein expression in the RAIC (A), dorsal hippocampus (B), PAG (C) and dorsal horn of spinal cord (D) of B6 mice. The B6 mice were injected with vehicle or morphine (10 mg/kg, i.v.) and sacrificed at the indicated time points (30 and 60 min). The expression levels of hnRNP K (red) and NeuN (green, a neuronal marker) were visualized using immunofluorescence assay. DAPI (blue) is used as a nuclear marker for orientation. Scale bars, 50 µm. (E) Quantification of hnRNP K-positive cells in the total cell population was presented as the mean ± SD. Statistical analysis was carried out using the one-way ANOVA with appropriate post hoc tests. *P < 0.05, **P < 0.01, ***P < 0.001 versus vehicle control group.