Fig 2: Predicted docking of FBP substrate in the active site of aldolase.Best-scoring ligand docking complexes for ALDOA in the A) turtle and B) rabbit (crystal structure 1zaiA), as well as, ALDOB in the C) turtle and D) rabbit (crystal structure 1fdjA). Docking was modeled using MOE Dock, employing Triangle Matcher as the placement method and the function London dG as the first scoring function.

Fig 3: Protein alignment of ALDOA and ALDOB.Full amino acid sequences of A) ALDOA and B) ALDOB from T. s. elegans compared to O. cuniculus and P. sinensis. Dashes replace amino acid residues identical with T. s. elegans; spacer dots indicate residues that are missing in the sequence. Alignment was prepared using DNAMAN (v.4) software.

Fig 4: Aldolase purification and expression from the liver of control and anoxic turtles.A) SDS-PAGE gel with Coomassie blue staining of samples taken at each stage of aldolase purification from normoxic turtles T. s. elegans. B) Effect of 20 h of anoxic submergence on relative ALDOA and ALDOB total protein expression demonstrated with immunoblotting on whole cell lysates from liver tissue. Histogram showing mean normalized protein expression. Data in histograms are means ± SEM, n = 4 independent trials with tissue from different animals. * - indicates significantly different from the corresponding control (P<0.05). C) Co-immunoprecipitation of ALDOA and ALDOB from turtle liver. Endogenous ALDOA was co-immunoprecipitated with endogenous ALDOB in control and 20 h anoxic conditions. Experimental IgG control was carried out using p130 primary antibody (Santa Cruz; Cat# sc-317).