Fig 2: Distinguishing the normal and cystic epithelial cells and immunohistochemical verification of multiple cellular origins in individual cyst. (A) t-SNE plots showing the origin of epithelial cells from ADPKD tissues. Cell clusters expressing PT, LOH/DT, and CD markers were labeled as green, blue and red respectively. (B) t-SNE plots showing ADPKD gene set scores of epithelial cells from ADPKD tissues. (C) t-SNE plots showing origin of epithelial cells from normal kidney tissues. Cell clusters expressing PT, LOH/DT, and CD markers were labeled as green, blue, and red respectively. (D) t-SNE plots showing ADPKD gene set scores of epithelial cells from normal kidney tissues. (E) Origins of epithelial cells with high ADPKD gene set scores in each sample. Cell clusters expressing PT marker, LOH/DT marker, and CD marker were highlighted in green, blue and red respectively. (F) Violin plots showing distributions of ADPKD gene set score in normal and ADPKD tissues. (G) Violin plots showing distributions of ADPKD gene set score in different samples. (H-I) Individual cyst could derive from two cell types: serial section staining showed distribution of proximal tubule marker CD10 and LOH marker SLC12A1 in the same cyst. t-distributed stochastic neighbor embedding, t-SNE; autosomal dominant polycystic kidney disease, ADPKD; proximal tubules, PT; loop of Henle, LOH; distal tubules, DT; collecting ducts, CD.